An improved method for the direct counting of basophil leucocytes from venous or capillary blood is described. The method is based upon the use of cetylpyridinium chloride to lyse the erythrocytes and render the muco-polysaccharides of the granule insoluble. Aluminium sulphate is used to mordant toluidine blue to the granules. Our knowledge of the role of the basophil leucocytes in disease has ...

Introduction: Odontogenic cysts are characterized by an expansile non–infiltrative growth through several mechanisms which include presence of mast cells in the capsule. The mast cells can be demonstrated histochemically as well as immunohistochemically. Aim and Objectives: The primary objective of the study was to analyze the presence and distribution of mast cells in radicular cyst, dentigero...

for histological study of buffalo's uterine tube, 10 genital organs of adult buffalos were sampled. general histology was taken out by h & e method, and special staining methods that used were respectively verhoeff, toluidine blue, and pas. this study revealed that elastic fibers exist in connective tissue of folds and wall of uterine tube and tunica muscularis, and also they exist relativ...

IgA vasculitis is a disease causing the antibody immunoglobulin A to collect in small blood vessels, which become inflamed and leak affecting people of all age groups. The mechanisms remain unclear; however, studies suggest involvement mast cells progress vasculitis. To best our knowledge, limited human have been reported literature with detailed analysis cell character number purpose study det...

Varicocele is one of the most common causes of primary male infertility. Pilea microphylla (PM) is being used as folk medicine. This study was aimed to investigate the effects of PM in a rat model of varicocele. A total of 30 male Wistar rats were divided into control, sham, varicocele, accessory varicocele and PM-treated groups. After 10 weeks of varicocele induction, sperm parameters and chro...

PURPOSE Neuropeptide-containing nerves can serve as a mechanism for nervous system regulation of host defense responses. Because bacteria associated with reactive arthritis have been identified in the temporomandibular joint (TMJ), this study investigates whether the presence of substance P (SP) neuropeptide-containing nerves and mast cells can be identified in the TMJ. MATERIAL AND METHODS P...

The monoclonal antibody (MoAb) Bsp-1 was used to purify basophilic cells from leukemic blood of five patients with Philadelphia chromosome (Ph') positive chronic myeloid leukemia (CML) and two patients with acute myeloid leukemia (AML) characterized by the chromosomal translocation t(6;9)(p23;q34). When cultured, Bsp-1 positive cells from all CML and AML patients showed the same clonal karyotyp...

Bronchoalveolar lavage was performed in 97 patients including control patients with bronchial carcinoma (24) and patients with sarcoidosis (20), cryptogenic fibrosing alveolitis (9), and asthma (4), and others. Cytocentrifuged slides were stained by two methods: May-Grünwald Giemsa and toluidine blue. In the last 32 subjects the bronchoalveolar lavage fluid was separated into supernatant and ce...

Biocompatibility testing for a new implant material is necessary before clinical use. The materials bone scaffolding must also go through biocompatibility tests both in vitro and vivo. purpose of this study was to compare different staining methods histological preparations assess the tissues. Twenty Wistar rats (n= 20), aged 4 months, males, weighing about 250-350 g were divided into 2 researc...

Current literature is lacking regarding the vegetative propagation of cambuci tree (Campomanesia phaea O. Berg Landrum). This study aimed to verify efficiency cutting techniques via assessment cuttings varying types and sizes, sampling dates, influence treatments containing plant growth regulator indolebutyric acid (IBA) combined with antioxidant ascorbic across different immersion times. The s...