Using restriction endonucleases which preferentially digest mouse main band DNA and leave satellite DNA intact, we have isolated highly purified chromatin fractions containing only mouse satellite or main band DNA. Following the digestion of mouse brain nuclei with EndoR Alu I, main band DNA chromatin is selectively extracted with 10mM Tris, 10mM EDTA. Satellite DNA chromatin is subsequently ex...

Salmonoid fish bdellosis is caused by leech-like ectoparasites in the monogenetic order Acanthobdellida. Although Acanthobdella species have been known to infect several threatened species in Eurasia, little is known about their ecology and epidemiology. In this paper, we report on the mass affection (up to 70.7%) of fish in lower course of the Chechuj River, a right tributary of the Lena and p...

Wakes caused by swimming goldfish (Carassius auratus) were measured with a particle image velocimetry system and analyzed using a cross-correlation technique. Particle velocities in a horizontal plane (size of measuring plane 24 cmx32 cm or 20 cmx27 cm) were determined, and the vorticity in the plane was derived from these data. The wake behind a swimming goldfish can show a clear vortex struct...

PURPOSE The main purpose of this study was to analyze the coordination, propulsion and non-propulsion phases in the 100 meter breaststroke race. METHODS Twenty-seven male swimmers (15.7 ± 1.98 years old) with the total body length (TBL) of 247.0 ± 10.60 [cm] performed an all-out 100 m breaststroke bout. The bouts were recorded with an underwater camera installed on a portable trolley. The swi...

A study was carried out to determine the relationship between linear body measurements and live weight in Landrace and Large White pigs reared under different management conditions in Zimbabwe. Data was collected for body length, heart girth, and live weight in 358 pigs reared under intensive commercial conditions. The stepwise multiple linear regression method was done to develop a model using...

The boundary layers of rainbow trout, Oncorhynchus mykiss [0.231±0.016 m total body length (L) (mean±s.d.); N=6], swimming at 1.6±0.09 L s-1 (N=6) in an experimental flow channel (Reynolds number, Re=4×105) with medium turbulence (5.6% intensity) were examined using the particle image velocimetry technique. The tangential flow velocity distributions in the pectoral and pelvic surface regions (a...

Two new species of the family Enchelidiidae Filipjev, 1918 were collected from marine sediments near Maemul Island in South Korea: a new species of Abelbolla Huang & Zhang, 2004 and a new species of Ledovitia Filipjev, 1927. Abelbolla maemulensis sp. nov. is characterized by its small size (1,493 × 38 µm, body length × maximum body diameter); the presence of a circular amphid; the gubernacular ...

The function of species has been recognized as critical for the maintenance of ecosystems within desired states. However, there are still considerable gaps in our knowledge of interspecific differences in the functional roles of organisms, particularly with regard to the spatial scales over which functional impact is exerted. This has implications for the delivery of function and the maintenanc...

Nuclei from a variety of human cell lines and tissues were digested with gradually increasing levels of DNase I. The DNA was then purified, treated with restriction enzymes and subjected to Southern blot hybridization using a cloned cDNA probe to 3-phosphoglycerate kinase (PGK) a housekeeping enzyme. At relatively high levels of DNase I, a specific, slightly sensitive site in chromatin sequence...

Interpreting the functional state of chromatin from the combinatorial binding patterns of chromatin factors, that is, the chromatin codes, is crucial for decoding the epigenetic state of the cell. Here we present a systematic map of Drosophila chromatin states derived from data-driven probabilistic modelling of dependencies between chromatin factors. Our model not only recapitulates enhancer-li...