نتایج جستجو برای: transformation efficiency

تعداد نتایج: 599784  

2000
Edgar G. Hertwich Edgar Hertwich

The ecological efficiency of industrial production has become the focus of a range of innovative and promising research and implementation activities. Eco-efficiency promises to achieve a less polluted environment at lower costs than pollution control and ensure human well-being with less detrimental effects than adaptation and remediation. At the same time, it is less radical and therefore off...

2013
Madhavi Sonane Ritu Goyal Debapratim K. Chowdhuri Kristipati Ravi Ram Kailash C. Gupta

The efficiency of genetic transformation technology to generate stable transgenics depends upon the successful delivery of plasmid DNA in embryonic cells. The available gene vectors facilitate efficient plasmid DNA delivery to the cellular milieu but are exposed to nuclease degradation. Recent in vitro studies suggest encapsulation of plasmid DNA with nanomaterial(s) for better protection again...

2009
Kazumasa Yasui Yasunobu Kano Kaori Tanaka Kunitomo Watanabe Mariko Shimizu-Kadota Hirofumi Yoshikawa Tohru Suzuki

We have developed a method to improve the transformation efficiency in genome-sequenced bacteria, using 'Plasmid Artificial Modification' (PAM), using the host's own restriction system. In this method, a shuttle vector was pre-methylated in Escherichia coli cells, which carry all the putative genes encoding the DNA modification enzymes of the target microorganism, before electroporation was per...

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