Transcription termination is a critical step for gene regulation and genome integrity among all kingdoms of life. In <i>Saccharomyces cerevisiae</i>, one the major pathways accomplished by Sen1 helicase, homolog to human Senataxin (SETX), defection which raises diseases central nervus system human. Although it has been proposed that translocates along nucleic acids consuming adenosi...

BACKGROUND Little is known about the biological behavior of Xp11.2 translocation renal cell carcinomas (RCCs) as few clinical studies have been performed using a large sample size. METHODS This study included 103 consecutive young adult patients (age ≤ 45 years) with RCC who underwent partial or radical nephrectomy at our institution from 2008 to 2013. Five patients without complete clinical ...

Complexes formed between the bacteriophage phi29 DNA polymerase (DNAP) and DNA fluctuate between the pre-translocation and post-translocation states on the millisecond time scale. These fluctuations can be directly observed with single-nucleotide precision in real-time ionic current traces when individual complexes are captured atop the α-hemolysin nanopore in an applied electric field. We rece...

In cells, helicase translocation along nucleic acid is essential for many biological processes. However, so far, the mechanism of this translocation is not fully understood. Recent studies show that helicase might translocate through two processes, active process and passive process, with different translocation rate. In this study, a mechanochemical model including such two processes is presen...

We investigate single-molecule electrophoretic translocation of A(50), C(50), A(25)C(50), and C(50)A(25) RNA molecules through the alpha-hemolysin transmembrane protein pore. We observe pronounced bilevel current blockages during translocation of A(25)C(50) and C(50)A(25) molecules. The two current levels observed during these bilevel blockages are very similar to the characteristic current lev...

The alpha isoform of protein kinase C (PKCalpha) is a ubiquitous protein kinase, which, upon activation, translocates rapidly from the cytoplasm to the plasma membrane. To follow this translocation, PKCalpha was tagged with a highly fluorescent derivative of green fluorescent protein and stably expressed in baby hamster kidney cells overexpressing the muscarinic type 1 receptor. Addition of the...

ABSTRACT Chromium contamination in soils affects plant growth and this metal can accumulate plants tissues. In addition, Cr affect soil microbial biomass activity. On the other hand, growth-promoting rhizobacteria (PGPR) protect against metals and, at same time, promote could alleviate adverse effects on biomass. This study evaluated five PGPR maize growth, accumulation a Cr-contaminated soil. ...