Cancer metabolism and epigenetic alteration are two critical mechanisms for tumorigenesis and cancer progression; however, the dynamic interplay between them remains poorly understood. As reported in the article entitled "Chromatin remodeling factor LSH drives cancer progression by suppressing the activity of fumarate hydratase," which was recently published in Cancer Research, our group examin...

It has been proposed that enzymes in many metabolic pathways, including the tricarboxylic acid cycle in the mitochondrial matrix, are physically associated to facilitate substrate channeling and overcome diffusive barriers. We have used fluorescence recovery after photobleaching to measure the diffusional mobilities of chimeras consisting of green fluorescent protein (GFP) fused to the C termin...

Studies on the metabolism of the strict aerobe Micrococcus sodonensis revealed that a considerable accumulation of a-ketoglutarate and glutamate occurred. This accumulation was in evidence, both during growth on complex media and during growth on chemically defined media with acetate as the sole carbon source. The major pathway of terminal respiration in aerobically growing microorganisms appea...

Rats were infused with glucose at 30 mg/min, containing 18% enriched [U-13C]glucose and [1-14C]- and [3-3H]glucose. The mass isotopomer patterns of 13C-labeled blood glucose and liver glycogen were determined by gas chromatography/mass spectroscopy. The contribution of the direct pathway to glycogen was calculated from the three tracers, and the values by all three were nearly identical, about ...

The first branch point in gluconeogenesis occurs at the conversion of pyruvate to oxaloacetate. To determine the amount of lactate carbon reaching glucose via the direct pyruvate carboxylase pathway versus the tricarboxylic acid cycle, adult rat hepatocytes in primary culture were incubated for 2 h with one of the following isotopic substrates: [1-14C]lactate, [U-14C]lactate, or [1,2-14C]acetat...

For many years the importance of the Krebs tricarboxylic acid cycle in bacterial metabolism has been contested. Some authors find evidence for the occurrence of the cycle in certain species of bacteria; in other cases, results are interpreted as proving that the cycle does not operate (see Ajl for a recent review (1)). In most of these studies, as indeed with those on other organisms, the empha...

Evidence has been presented (Saz and Krampitz, 1954) which demonstrates that a-ketoglutarate and citrate are intermediates on the pathway of acetate oxidation by partially lysed cell preparations of Micrococcus lysodeikticu8. Results reported in this publication extend and further substantiate our previous findings. Despite the fact that condensing enzyme can be demonstrated in many aerobic bac...

Studies on the metabolism of the strict aerobe Micrococcus sodonensis revealed that a considerable accumulation of a-ketoglutarate and glutamate occurred. This accumulation was in evidence, both during growth on complex media and during growth on chemically defined media with acetate as the sole carbon source. The major pathway of terminal respiration in aerobically growing microorganisms appea...

In this work, we studied the optimization of synthetic approaches to creating structurally modified metal-organic frameworks under various synthesis conditions. We investigated influence solvents and zinc salts on structural characteristics framework based benzene-1,3,5-tricarboxylic acid (H3BTC). The results indicate that variation types both solvent salt is a parameter affecting crystallinity...