Genistein (GEN) is one of the predominant dietary isoflavones found in legumes such as soybeans. has been recommended an osteoporosis treatment for postmenopausal women and elderly men, with intention reducing cardiovascular disease hormone-dependent malignancies. Therefore, two sensitive simple methods quantifying it supplements preparation were developed.The first method (A) comprised employi...

We examined the subcellular location of bactericidal factors (BF) in human neutrophils, using an efficient fractionation scheme. Nitrogen bomb cavitates of DIFP-treated PMN were centrifuged through discontinuous Percoll gradients, each fraction extracted with 0.05 M glycine, pH 2.0, and tested for the killing of Escherichia coli. greater than 90% of BF coisolated with the azurophil granules. Af...

The cloud point extraction behavior of aluminum(III) with 8-quinolinol (HQ) or 2-methyl-8-quinolinol (HMQ) and Triton X-100 was investigated in the absence and presence of 3,5-dichlorophenol (Hdcp). Aluminum(III) was almost extracted with HQ and 4(v/v)% Triton X-100 above pH 5.0, but was not extracted with HMQ-Triton X-100. However, in the presence of Hdcp, it was almost quantitatively extracte...

Triton X-100 is a neutral detergent which is often employed for reconstitution of lipidprotein membranes. It is, however, difficult to remove entirely from the final reconstituted membrane preparation. We have analyzed the molecular components of the Triton X-100 complex of molecules that remain in reconstituted membranes which are prepared by, first, Triton X-100 solubilization and, second, by...

A cloud point extraction process using the nonionic surfactant Triton X-114 for extracting mercury from aqueous solutions was investigated. The method is based on the complexation reaction of Hg(II) with Thio-Michler's Ketone (TMK) and micelle-mediated extraction of the complex. The optimal extraction and reaction conditions (e.g., pH, reagent concentration, effect of time) were studied, and th...

The apicomplexan parasite, Theileria annulata, is the causative agent of tropical theileriosis, a devastating lymphoproliferative disease of cattle. The schizont stage transforms bovine leukocytes and provides an intriguing model to study host/pathogen interactions. The genome of T. annulata has been sequenced and transcriptomic data are rapidly accumulating. In contrast, little is known about ...