compounds containing triazene ring structure are cytotoxic agents and clinically used as antitumor alkylating agents. in this study, a series of triazene derivatives holding alkyl and aryl moieties were synthesized and proved to be potent cytotoxic agents in-vitro particularly against eight cancer cell lines (pc3, ht29, hela, hl60, jurkat, k562, mcf7, hepg2) and a non-cancerous cell line (huvec...

background: electroporation is a valuable tool for small interfering rna (sirna) delivery into cells because it efficiently transforms a wide variety of cell types. since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect mda-mb 468 human breast cancer cell with sirna. ...

Background: Electroporation is a valuable tool for small interfering RNA (siRNA) delivery into cells because it efficiently transforms a wide variety of cell types. Since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect MDA-MB 468 human breast cancer cell with siRNA. ...

Through in vitro experiments, the paper investigated whether Lycopene can mitigate cardiac ischemia/reperfusion injury, and further explored underlying mechanism works by inhibiting opening of MPTP.A hypoxia-reoxygenation model was established. CCK8 employed to determine viability H9c2 cardiomyocytes. LDH used degree damage Trypan blue staining applied survival rate Flow cytometry apoptosis Rho...

The effect of muscimol on N-methyl-D-aspartate (NMDA)-induced injury of primary cultured cerebral cortical neurons was examined. NMDA induced a dose-dependent leakage of LDH activity, which was significantly inhibited by (+-)-5-methyl-10,11-dihydro-5H-dibenzo-[a,d]cyclopentan-5,10-imine (MK-801). Muscimol significantly reduced the NMDA-induced increase of lactic dehydrogenase (LDH) leakage, and...

A 50 year old man presented to Eye clinic University clinical centre Tuzla with bilateral visual impairment. Clinical examination revealed low visual acuity and keratoconus in both eyes, white cataract in right eye and diabetic retinopathy in left eye. Ultrasonography examination was normal. The patient underwent Trypan blue capsule staining, phacoemulsification and implantation of intraocular ...