An anionic trypsinogen has been identified and isolated in a state of high purity from porcine pancreatic tissue and juice extracts. The anionic trypsinogen is present in large amounts and accounts for about half of the total potential tryptic activity. The kinetics of the conversion of the anionic trypsinogen to active enzyme is autocatalytic, yielding an anionic trypsin which is less anionic ...

Human lymphocytes were grown in short-term tissue culture and were arrested in metaphase with Colcemid. Their chromosomes were prepared by the Langmuir trough-critical point drying technique and were examined under the electron microscope. In addition, some chromosomes were digested with trypsin, Pronase, or DNase. The chromosomes consist entirely of tightly packed, 240 +/- 50-A chromatin fiber...

In this study, we examined the use of multiple proteases (trypsin, LysC, tandem LysC/trypsin) on both protein identification and quantification in the Lys-labeled SILAC mouse liver. Our results show that trypsin and tandem LysC/trypsin digestion are superior to LysC in peptides and protein identification while LysC shows advantages in quantification of Lys-labeled proteins. Combination of exper...

Trypsin is known to activate certain proenzymes, including trypsinogen and chymotrypsinogen.7 It also activates prothrombin." In the case of prothrombin activation, platelets have been shown to complement the effect of trypsin. Small quantities of trypsin and platelets together exert a much greater effect than the sum of their separate effects.9 Lipoid thromboplastin has been found to complemen...

I have shown that cyclic AMP stimulates sugar uptake in rat thymocytes. However, trypsin treatment, which increases rat thymocyte cyclic AMP concentration, fails to increase sugar uptake. The purpose of the present study is to examine this seeming inconsistency, and to evaluate further the function of trypsin. Mild trypsin treatment of rat thymocytes produced a dose-related increase in cellular...

How glycosylation affects the reactivity of proteins to trypsin is not well understood. Bovine and porcine pancreatic trypsins were discovered to bind to alpha-Man, Neu5Acalpha2,6Galbeta1,4Glc, and alpha-galactose sequences by binding studies with biotinylated sugar-polymers. Quantitative kinetic studies supported that phenylmethylsulfonyl fluoride (PMSF)-treated trypsin binds to glycolipid ana...

Colostrum samples from 49 Jersey cows were analyzed for concentrations of trypsin inhibitor, IgG, IgM, IgA, TS, fat, specific gravity, and N fractions. Colostrum (100 ml) was sampled from each cow as soon as possible after parturition. Mean concentrations of IgG, IgM, and IgA were 84.6, 3.4, and 4.5 g/L, respectively. Mean concentration of trypsin inhibitor was 56 mg of trypsin inhibited/dl of ...

Human mesotrypsin is an isoform of trypsin that displays unusual resistance to polypeptide trypsin inhibitors and has been observed to cleave several such inhibitors as substrates. Whereas substitution of arginine for the highly conserved glycine 193 in the trypsin active site has been implicated as a critical factor in the inhibitor resistance of mesotrypsin, how this substitution leads to acc...