The highly efficient nature of dendritic cells (DC) as antigen-presenting cells raises the possibility of uncovering in tumor-bearing hosts very low levels of T cell reactivity to poorly immunogenic tumors that are virtually undetectable by other means. Here, we demonstrate the in vitro and in vivo capacities of murine bone marrow-derived, cytokine-driven DC to elicit potent and specific anti-t...

Recently, casein kinase II was suggested to play a role in accurate transcription in vitro (R. Zandomeni, M. C. Zandomeni, D. Shungar, and R. Weinmann, J. Biol. Chem., 261, 3414 (1986)). In the present study, we examined whether transcription factor S-II is a target of casein kinase II, because the phosphorylated form of S-II, termed S-II', is known to be present in vivo. We found that S-II was...

background: noradrenaline (na), the principal neurotransmitter released from sympathetic nerve terminals, influences t-cell maturation, not only directly in developing t cells, but also indirectly, by acting on the thymic nonlymphoid cells. in vitro and in vivo studies have demonstrated the anti-proliferative, anti-migratory, antiangiogenic and cytotoxic properties of propranolol, β-ar blocker,...

objective(s): in order to grow cells in a three-dimensional (3d) microenvironment, self-assembling peptides, such as puramatrix, have emerged with potential to mimic the extracellular matrix. the aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma st...

Platelet aggregation activity due to platelet-activating factor (PAF) was detected at high molecular weight (HMW) and low molecular weight fractions after gel-filtration chromatography of cell lysate of endothelial cells. [3H]PAF added to the cell lysate was similarly distributed after chromatography. The radioactivity associated with HMW fraction was not reduced by digesting the lysate with tr...

To determine whether pathogenic Acanthamoeba culbertsoni trophozoites and lysate can induce cytopathic changes in primary-culture microglial cells, morphological changes were observed by transmission electron microscopy (TEM). In addition, the secretion of two kinds of cytokines, tumor necrosis factor alpha (TNF-alpha) and interleukin-1beta (IL-1beta), from microglial cells was observed. Tropho...

ovarian mixed germ cell tumors consisting of endodermal sinus tumor and immature teratoma are very rare and have been reported only in a few case reports. here we report a rare mixed germ cell tumor of ovary which was consisted of endodermal sinus tumor and immature teratoma components with an unusual intraabdominal location. patient was a 21 years old girl with the chief compliant of abdominal...

"Day-7" myeloid DCs are commonly used in the clinic. However, there is a strong need to develop DCs faster that have the same potent immunostimulatory capacity as "Day-7" myeloid DCs and at the same time minimizing time, labor and cost of DC preparations. Although "2 days" DCs can elicit peptide-specific responses, they have not been demonstrated to engulf, process and present complex whole tum...

background: giant cell tumor is a benign but locally aggressive bone neoplasm which uncommonly involves the skull. giant cell tumor (gct) of the sphenoid bone is relatively rare. in this report, we describe a tumor of the greater wing of left sphenoid bone. case presentation: a 31-year-old female was presented with headache, proptosis and diplopia of her left eye. cranial magnetic resonance ima...