Non ionic surfactant vesicles (niosomes) composed of sorbitan monostearate (Span 60), polyoxyethylene sorbitan monostearate (Tween 61), cholesterol, and dicetyl phosphate were conjugated with a purified monoclonal antibody to CD44 (IM7) through a cyanuric chloride (CC) linkage on the polyoxyethylene group of the Tween 61 molecule. Inclusion of small amounts of Tween 61 within the surfactant com...

Chitosan, a natural biodegradable polymer is recommended for development of nanoparticles intended for chronic infection such as tuberculosis. Formulation and processing variables play a critical role in the formation of nanoparticles prepared with chitosan. The present study investigated the influence of polymer (chitosan) molecular weight, stabilizing agent (tween 80) concentration and stirri...

The surface properties of cationic liposomes and lipoplexes largely determine the cellular association and gene transfection efficiency. In this study, we measured the surface properties, such as zeta potentials, surface pH and hydration levels of MHAPC- and OH-Chol-lipoplexes and their cellular association, without and with the modification of biosurfactant mannosylerythritol lipid-A (MEL-A) o...

Docetaxel is a potent anticancer drug, but development of an oral formulation has been hindered mainly due to its poor oral bioavailability. In this study, solid lipid nanoparticles (SLNs) surface-modified by Tween 80 or D-alpha-tocopheryl poly(ethylene glycol 1000) succinate (TPGS 1000) were prepared and evaluated in terms of their feasibility as oral delivery systems for docetaxel. Tween 80-e...

Attempts were made to solubilize thymol in Tween 80 micelle to study the solubilization mechanism of thymol and the effect of solubilization on its antioxidant activity. The maximum solubilized concentration of thymol in a 2.0% (w/v) Tween 80 micelle solution is 0.2 wt%. There was no significant difference in Z-average diameter between the empty micelles and thymol solubilized micelles. 1H NMR ...

It has been difficult to cultivate human tubercle bacilli in agar medium unless bovine serum, or a fraction therefrom, was added aseptically, since the factor present in the serum is thermolabile (Dubos and Middlebrook, 1947). On the other hand, egg yolk or whole egg media may be used, but the difficulties in preparing the media render the methods uncertain and cumbersome. Hence, a thermostable...

Optical devices for measuring protoporphryin IX (PpIX) fluorescence in tissue are routinely validated by measurements in optical phantoms. Yet there exists limited data to form a consensus on the recipe for phantoms that both mimic the optical properties found in tissue and yield a reliable and stable relationship between PpIX concentration and the fluorescence remission intensity. This study c...

In this study, the effects of the addition of Tween 80 and acetone on secretion, structure and antioxidant activities of Lentinus tigrinus exopolysaccharides (EPS) were investigated. It was found that Tween 80 and acetone displayed a stimulatory effect on EPS secretion. The EPS obtained by the addition of Tween 80 (EPS-T), acetone (EPS-A) and control (EPS--C) were purified by Sepharose CL-6B ge...

Self-emulsifying drug delivery systems (SEDDS) were prepared by dissolving Cremophor EL, Tween 20, Tween 80 and Span 80 (1% or 5%) in oils (Miglyol 812 or castor oil). Solubilities of three ophthalmic drugs, namely aciclovir, hydrocortisone and indomethacin were determined in these systems. In addition, the effect of a small amount of water (0.5% and 2%) on solubilization properties of the syst...

The fatty acid composition of animal cells cultured in serum-free medium can be manipulated when the synthesis of endogenous fatty acids is inhibited by a biotin analog and fatty acids are supplied in the medium as detergent esters of Tween. When mouse LM cells were grown in medium supplemented with Tween-19:0 (an ester of Tween and nonadecanoic acid), odd chain fatty acid content of cellular p...