Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.

The amount of 140 individual proteins of E. coli B/r was measured during balanced growth in five different media. The abundance of each protein was determined from its absolute amount in 14C-glucose-minimal medium and a measurement of its relative amount at each growth rate using a double labeling technique. Separation of the proteins was carried out by two-dimensional gel electrophoresis. This...

A new method for spot matching in two-dimensional gel electrophoresis images using a cumulative frequency matrix is proposed. The method improves on the weak points of the previous method called 'spot matching by topological patterns of neighbour spots'. It accumulates the frequencies of neighbour spot pairs produced through the entire matching process and determines spot pairs one by one in or...

A Landau-de Gennes model that integrates the nematic quadrupolar tensor order parameter and complex smectic-A order parameters is used to simulate the two-dimensional growth of an initially homogeneous smectic-A spherulite in an isotropic matrix. These simulations are performed in the shape-dynamic (nano-scale) regime of growth under two material conditions: isotropic nematic elasticity and equ...

Granular materials are predominantly plastic, incrementally nonlinear, preparation-dependent, and anisotropic under shear. Nevertheless, their static stress distribution is well accounted for, in the whole range up to the point of failure, by a judiciously tailored isotropic nonanalytic elasticity theory termed granular elasticity. The first purpose of this paper is to carefully expound this vi...

exact solution for steady two-dimensional flow of an incompressible magma is obtained. themagmatic flow is studied by considering the magma as a second grade fluid. the governing partialdifferential equations are transformed to ordinary differential equations by symmetry transformations. resultsare discussed through graphs to understand the rheology of the flowing magma

Abstract This note aims at illustrating the application of Virtual Element Method to elasticity problems in mixed form, following Hellinger-Reissner variational principle. In order highlight potential and flexibility our approach, we focus on a three-dimensional low-order scheme, but similar considerations apply two-dimensional higher-order methods.

A subset W of the vertices of a graph G is a resolving set for G when for each pair of distinct vertices u,v in V (G) there exists w in W such that d(u,w)≠d(v,w). The cardinality of a minimum resolving set for G is the metric dimension of G. This concept has applications in many diverse areas including network discovery, robot navigation, image processing, combinatorial search and optimization....

In this paper an overview of functionally graded materials and constitutive relations of electro elasticity for three-dimensional deformable  solids is presented, and  governing equations of the Bernoulli–Euler and Timoshenko beam theories which account for through-thickness power-law variation of a two-constituent material and piezoelectric layers are developed  using the  principle  of virtua...