Cleaning validation (CV) is a Good Manufacturing Practices (GMP) requirement in manufacturing of drug substances and drug products. Liquid Chromatography with UV detection is a common choice for cleaning verifi cation studies for most drugs at sensitivity levels of approximately 20–50 ng/mL. For highly potent drugs, CV methods to reach sensitivity levels of low ng/mL can be implemented using UV...

Liquid chromatography coupled with tandem mass-spectrometry has remarkably enhanced the qualitative and quantitative analysis of pesticide residues in agriculture matrices. The matrix effects may either suppress or enhance responses target analytes on UPLC-MS/MS cause a reduction accuracy, precision sensitivity an analytical method. Thus, Here performed impact interferences tomato okra QuEChERS...

Purpose: To study the metabolites of phloretin in vivo using ultra-high performance liquid chromatography linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap).
 Methods: After administration (50 mg/kg; oral route) to six rats, blood samples were taken from each animal. Each sample was then subjected solid-phase extraction prepare it for chromatographic/spectroscopic analysis. Fi...

Vegetables and fruits can potentially accumulate cyanotoxins after water contaminated with cyanobacteria is used for irrigation. We developed validated an analytical method to quantify eight microcystin congeners (MCs) nodularin (NOD) using ultra high-performance liquid chromatography coupled tandem mass spectrometry (UHPLC-MS/MS) in three different matrices. Strawberries, carrots lettuce are s...

An efficient and rapid affinity-based screening method for directly fishing out natural alpha-glucosidase inhibitors from Cyperus. rotundus extract by using immobilized enzyme technology combined with UHPLC-QTOF MS analysis was established. As a results, without time-consuming and laborious isolation workload and false positive interference, five natural alpha-glucosidase inhibitors were succes...

Chromatographic techniques such as GC and HPLC are traditionally used for the analysis classification of olive oil, while NMR is another powerful technique. In this paper we first time to classify oil produced by four cultivars, namely ‘Arbequina’, ‘Arbosana’, ‘Koroneiki’, ‘Sikitita’, planted in super-high-density frames California, United States. We compared results with targeted fatty acids t...

A sensitive method is introduced to detect selected pharmaceutical residues and polar pesticides with UHPLC-MS in water samples of different origin. Active or passive water sampling was combined with a laboratory solid-phase extraction cleanup and stable isotope dilution analysis. Recovery experiments demonstrated that the internal standard correction performed well for the compensation of matr...

Bosentan monohydrate (4-tert-butyl-N-[6-(2-hydroxyethoxy)-5-(2-methoxyphenoxy)-2-(pyrimidin-2-yl) pyrimidin-4-yl]benzene-1-sulfonamide monohydrate) is a dual endothelin receptor antagonist (ERA) applied in the treatment of pulmonary arterial hypertension. To achieve effective process control of the bosentan monohydrate synthesis, it was necessary to develop a selective and not highly time-consu...

Chitinases and thaumatin-like proteins are important grape proteins as they have a great influence on wine quality. The quantification of these proteins in grape juices and wines, along with their purification, is therefore crucial to study their intrinsic characteristics and the exact role they play in wines. The main isoforms of these two proteins from Chardonnay grape juice were thus purifie...

Full scan field asymmetric waveform ion mobility spectrometry (FAIMS) combined with liquid chromatography and mass spectrometry (LC-FAIMS-MS) is shown to enhance peak capacity for omics applications. A miniaturized FAIMS device capable of rapid compensation field scanning has been incorporated into an ultrahigh performance liquid chromatography (UHPLC) and time-of-flight mass spectrometry analy...