نتایج جستجو برای: using rnease mini kit

تعداد نتایج: 3426840  

Journal: :Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan 2008
Rie Moriuchi Kimio Monma Kunihiro Kamata Akihiro Ibe

In order to evaluate three different methods for DNA extraction (CTAB, DNeasy Plant Mini Kit and Wizard DNA Clean-up Resin system), the yields of DNA extracted from soyproducts and the copy numbers of lectin genes amplified by quantitative PCR were compared. Fermented foods, such as miso and nattou, gave poor yields of DNA and low copy numbers with any method. However atsu-age and kinugoshi-tof...

Introduction: The Sc is an important parameter to monitor unit (MU) calculation in radiation therapy procedure. The aim of this study is to measurement and comparison of head scatter factor (Sc) for 9MV photon beam using the build-up cap and a columnar mini-phantom.   Materials and Methods: The measurements performed using a calibrated ion chamber (Scanditron...

2016
Irina N Riediger Alex R Hoffmaster Arnau Casanovas-Massana Alexander W Biondo Albert I Ko Robyn A Stoddard

Leptospirosis is a zoonotic disease usually acquired by contact with water contaminated with urine of infected animals. However, few molecular methods have been used to monitor or quantify pathogenic Leptospira in environmental water samples. Here we optimized a DNA extraction method for the quantification of leptospires using a previously described Taqman-based qPCR method targeting lipL32, a ...

2017
Kuo-Liang Yang Hsee-Bin Chen

OBJECTIVE We report here the human leukocyte antigen (HLA) allelic variety and haplotype composition in a cohort of the Taiwanese Chinese population and their patterns of linkage disequilibria on HLA-B: HLA-C alleles and HLA-DRB1: HLA-DQB1 alleles at a high-resolution level. MATERIALS AND METHODS Peripheral whole blood from 11,423 Taiwanese Chinese unrelated individuals was collected in acid ...

2009
Hiroshi Fukushima Kazunori Katsube Yoshie Tsunomori Ryoko Kishi Junko Atsuta Yuko Akiba

A set of four duplex SYBR Green I PCR (SG-PCR) assay combined with DNA extraction using QIAamp DNA Stool Mini kit was evaluated for the detection of foodborne bacteria from 21 foodborne outbreaks. The causative pathogens were detected in almost all cases in 2 hours or less. The first run was for the detection of 8 main foodborne pathogens in 5 stool specimens within 2 hours and the second run w...

2010
Paula García-Nogales Alicia Serrano Sonia Secchi Serafín Gutiérrez Anna Arís

Nucleic-acid based methods for bacterial identification are extremely useful in diagnostic applications due to their specificity and sensitivity. However, they require an optimal purification of the target molecules. As part of the development of a new diagnostic method for the detection of bacterial RNA in cow milk, we have compared four commercially available RNA extraction kits for the isola...

2016
L. Fraser P. Brym

The main objective of this study was to isolate high-quality total ribonucleic acid (RNA) from raw fresh semen and frozen-thawed boar semen, using a protocol comprising the conventional TRIzol assay and a membrane-based technique, the PureLink RNA mini kit. Bioanalyzer profile revealed that the sperm RNA size distributions comprised mainly intact RNA ranging from 1500 to 1800 bp, without any de...

Journal: :Annals of agricultural and environmental medicine : AAEM 2011
Joanna Hapunik Bronislava Víchová Grzegorz Karbowiak Irena Wita Marek Bogdaszewski Branislav Pet'ko

The main goal of our study was to determine the prevalence of Anaplasma phagocytophilum infections in wild cervids living in north-eastern part of Poland. Material used in the study was gathered between the years 2004- 2008. The blood samples from 106 red deer (Cervus elaphus), 32 sika deer (Cervus nippon hortulorum), 130 fallow deer (Dama dama) and 31 roe deer (Capreolus capreolus) were collec...

2010

Genotype/expression correlation in human pancreatic cDNAs. Isolated pancreatic islets were prepared by collagenase digestion and density gradient purification (1; 2). After isolation, islets were cultured free-floating in M199 culture medium (Sigma-Aldrich) at 5.5 mmol/l glucose concentration. Within 3 days of isolation, the islets were used for RNA extraction. Total RNA was extracted from the ...

صفری, حسین, علیپورراد, نسیبه,

 The large and small Coronal Mass Ejections (CMEs) and mini-CMEs are observed by the Extreme Ultra Violet Imager (EUVI) on the Solar Terrestrial Relation Observatory (STEREO). These eruption events are usually associated with dimming and wave-like brightenings. Here, the images of the full sun on 26 Apr 2008 with STEREO spacecraft (behind) with the cadences of 10 minutes and 150 seconds and pix...

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