Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is commonly used to obtain high resolution separation of complex mixtures of proteins. The method initially denatures the proteins that will undergo electrophoresis. Although covalent structural features of resolved proteins can be determined with SDS-PAGE, functional properties are destroyed, including the presence of non-cov...

To detect the outer membrane protein (OMP), which plays a key role in carbapenem resistance, whole-genome and transcriptome analysis of the clinical carbapenem-resistant Klebsiella pneumoniae was carried out. The index strain lacked both OmpK35 and OmpK36, whereas the other strains lacked only OmpK35. After SDS-PAGE, the putative OMP bands were excised and identified as OmpA and OmpK36. MALDI-T...

Batch tests were conducted using four groundwater samples high levels of total hardness such as 600.17, 800.42, 1088.85 and 1278.38 mg/L as CaCO3, so as water is not suitable for several industries. The work aimed to evaluate the feasibility of using powdered pseudoactivated carbon (PAC) prepared from wheat husk for the removal of water hardness and also to study of impact of sodium dodecyl sul...

Detergents have several biological applications but present cytotoxicity concerns, since they can solubilize cell membranes. Using the IonFlux 16, an ensemble whole cell planar patch clamp, we observed that anionic sodium dodecyl sulfate (SDS), cationic cetyltrimethylammonium bromide (CTAB), and cationic, fluorescent octadecyl rhodamine B (ORB) increased the membrane permeability of cells subst...

background and objectives: sodium dodecyl sulfate (sds) is one of the most widely used anionic detergents. the present study deals with isolation and identification of sds-degrading bacteria from a detergent contaminated pond situated in varanasi city, india. materials and methods : employing enrichment technique in minimal medium (pbm), sds-degrading bacteria were isolated from pond water samp...

Poly(methylmethacrylate) (PMMA) microchip electrophoresis of sodium dodecyl sulfate-protein complexes (SDS-PC) using linear-poly(acrylamide) (L-PA) as a separation matrix was investigated. Prior to electrophoresis, channel walls of PMMA were modified with methylcellulose (MC) to prevent adsorption between channel walls and SDS-PC. Size-based protein separation (SBPS) was successfully performed ...

There has been minimal research into how to best obtain DNA from touch samples. Many forensic laboratories simply moisten a swab with water and use it for collecting cells/DNA from evidentiary samples. However, this and other methods have not been objectively studied in order to maximize DNA yields. In this study, fingerprints were collected using swabs moistened with water or laboratory or com...

The in-plane rotational dynamics of the molecular probe coumarin 314 (C314) at the negatively charged surfactant sodium dodecyl sulfate (SDS)/aqueous interface was measured using femtosecond time-resolved second-harmonic spectroscopy. The in-plane orientational time constant at an SDS surface coverage of 100 Å2 per SDS molecule is 348 ( 12 ps, which is comparable to the out-of-plane reorientati...

BACKGROUND Responsiveness to GH treatment can be estimated by both growth and ∆IGF-I. The primary aim of the present study was to investigate if mimicking the physiological increase during puberty in GH secretion, by using a higher GH dose could lead to pubertal IGFs in short children with low GH secretion. The secondary aim was to explore the relationship between IGF-I, IGFBP-3 and the IGF-I/I...

In this study, we established a rapid and sensitive method for the detection of viable Salmonella Typhimurium, Staphylococcus aureus, Listeria monocytogenes in milk using biotin-exposure-based immunomagnetic separation (IMS) combined with sodium dodecyl sulfate (SDS), propidium monoazide (PMA), multiplex real-time PCR (mRT-PCR). We used IMS to lessen assay time isolation target bacteria. then o...