Leptospires have never been recovered from goats in Brazil. Serum samples were obtained from 248 goats from Rio de Janeiro and from the seroreactive animals, urine samples were collected and processed for Leptospira isolation. A total of 52 positive reactions were observed, corresponding to 20.9% of the samples. The most prevalent reactions were to serovars Hardjo (36.5%), Shermani (30.8%), Ict...

Two hundred and seventy-five serum samples from cattle in Malawi were tested as a pilot survey for Leptospira antibody titres. Fifty-nine (21.4%) of the animals were positive for leptospirosis, while 35 (12.7%) animals reacted inconclusively. Titres to L. hardjo and L. pomona serovars were the most prevalent. Results are also discussed with reference to the areas where samples were collected.

The aim of the study was to determine the seroprevalence of Leptospira spp. and Borrelia burgdorferi sensu lato in healthy horses living in 7 provinces of central Italy. In the period 2007-2009, sera from 386 horses were tested by microagglutination test (MAT) to detect antibodies to Leptospira spp., employing the following serovars as antigens: Bratislava, Ballum, Canicola, Icterohaemorrhagiae...

Blood and feces were collected from 34 adult (19 males, 15 females) and seven juvenile (three males, one female, three not reported) free-ranging coyotes (Canis latrans) on the US Department of Energy's Savannah River Site (South Carolina, USA). Significant (P<0.05) hematologic differences by sex were noted for red blood cell counts, hemoglobin, and hematocrit. Biochemical differences by sex oc...

Leptospirosis is an infectious, zoonotic disease of a major worldwide importance of animals and humans (1, 2). It is caused by antigenically distinct serovars of the spirochetal bacterium Leptospira interrogans sensu lato. Over 200 serovars of L. interrogans sensu lato have been described and have been classified in different species (e.g., L. interrogans, L. kirschneri, L. borgpetersenii) (1-5...

This work reports a survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutination test (MAT) (n = 51) and by both techniques (n = 47). PCR detected infections in two pampas de...

We compared random amplified polymorphic DNA (RAPD) fingerprinting with cross-absorption agglutination and restriction enzyme analysis for typing bovine leptospires. Using RAPD fingerprinting, we examined a number of Leptospira serovars, namely, hardjo genotypes bovis and prajitno, pomona, balcanica, tarassovi, swajizak, kremastos, australis, and zanoni, which are likely to be isolated from Aus...

Abstract Leptospirosis is an endemic zoonotic disease that distributed worldwide, which has the potential to have health and economic impacts. Leptospira spp. spiral-shaped capable of infecting mammals, including horses, may result in asymptomatic or clinical forms. Therefore, current study aimed analyze frequency anti-Leptospira antibodies serum samples from horses Santarém, Pará, Brazil. For ...

BACKGROUND Leptospirosis in dogs is a disease of global importance. Early detection and appropriate therapeutic intervention are necessary to resolve infection and prevent zoonotic transmission. However, its diagnosis is hindered by nonspecific clinical signs and lack of rapid diagnostic tests of early infection. Recently, 2 rapid point-of-care tests (WITNESS Lepto [WITNESS Lepto, Zoetis LLC, K...

Leptospira interrogans serovar lai was identified in China in 1966 as a new serovar of the icterohaemorrhagiae serogroup by cross-absorption tests. In this study, we established three hybridoma cell lines producing monoclonal antibodies (MAbs) of the immunoglobulin G3 subclass (LW1, LW2, and LW3) and of the immunoglobulin M class (LW4a) against serovar lai strain 017 by the cell fusion techniqu...