the field of vaccinology provides excellent promises to control different infectious and non-infectious diseases. genetic immunization as a new tool in this area by using naked dna has been shown to induce humoral as well as cellular immune responses with high efficiency. this demonstrates the enormous potential of this strategy for vaccination purposes. dna vaccines have been widely used to de...

Letters tion centers in Lagos was fully potent, but potency in Osun and Oyo was 016 log 10 to 0.22 log 10 lower than the stipulated level (2). Furthermore, the titer of two vaccine lots that had been frozen after reconstitution from their lyophilized state dropped from the initial 3.15 log 10 to 3.53 log 10 to zero. If the United States were to implement an extended strategy, similar studies of...

Administration of a birth dose of hepatitis B vaccine (HepB vaccine) to neonates is recommended to prevent mother-to-infant transmission and chronic infection with the hepatitis B virus (HBV). Although manufacturers recommend HepB vaccine distribution and storage at 2-8 degrees C, recognition of the heat stability of hepatitis B surface antigen stimulated research into its use after storage at,...

The European Pharmacopoeia (Ph. Eur.) and the World Health Organization (WHO) require the performance of extensive quality control testing including a potency test before a vaccine batch is released for human use. Whole cell pertussis (wP) vaccine potency is assessed by a mouse protection test (MPT) based on the Kendrick test. This test compares the vaccine dose necessary to protect 50% of mice...

Background: Improving vaccine potency in the induction of a strong cell-mediated cytotoxicity can enhance the efficacy of vaccines. Necrotic cells and the supernatant of necrotic tumor cells are attractive adjuvants, on account of their ability to recruit antigen-presenting cells to the site of antigen synthesis as well as its ability to stimulate the maturation of dendritic cells. Objective: T...

A formalin-inactivated Eastern equine encephalomyelitis vaccine was prepared from the PE-6 strain virus, propagated in primary chick-embryo cell cultures maintained with Medium 199 containing 0.25% human serum albumin. To effect an increase in antigenic mass per unit volume of fluid harvest, we used the pooled fluids from the first tissue-culture passage as maintenance medium for two additional...

In the present study, the potency and immunogenicity of the inactivated bivalent vaccine of Newcastle disease (ND) and avian influenza (AI) produced by Razi institute in Iran were compared with a similar imported vaccine administered by standard methods to specific-pathogen-free (SPF) chicken. A total of 150 twenty-one-day-old SPF chickens were used for evaluating Razi and imported inactivated ...

1. The principle of the N I H test is the immunisation of mice with different vaccine concentrations, followed by challenge infection with one virus concentration of 5 to 50 LD50/dose of the challenge virus strain (CVS) derived from the Pasteur strain. The protection rates ( E D 5 0 ) of a standard and the test vaccines are calculated, and the potency of the test vaccine is given in Internation...