Unlike the development of drugs based on small chemical entities there are no conventional regulatory toxicity studies established for DNA-based products. As the potential for insertional mutagenesis is of particular concern for gene therapy, we have investigated the mutagenicity of model non-viral DNA-based products at the HPRT locus in Chinese hamster V79 cells. Cultures were transfected with...

SARS-CoV-2 is the infectous agent of Covid-19, one most important health problems twenty-first century. IL-17RA an crucial receptor in generation host immune response. ORF8 viral accessory protein that suppresses Mutations can alter properties and clinical course SARS-CoV-2. In this study, we investigated changes mutations may cause interaction with ORF8. The study was carried out using 825 com...

A frequent mutation at codon 97 of human hepatitis B virus core antigen has been shown to cause an "immature secretion" phenotype, featuring nonselective and excessive secretions of virions containing immature viral genome. Our current study demonstrates that this abnormality can be efficiently offset by another frequent core mutation, P130T.

objective(s): it has been reported that the mutation of the pre-core (pc) and basal-core promoter (bcp) may play an important role in the development of hbv-related hepatocellular carcinoma (hcc). in this study the pc and bcp mutations were investigated in chronic hbv patients. materials and methods:in this study, 120 chronic hbv patients from golestan, northeast of iran who were not vaccinated...

A mutation in the Ebola virus glycoprotein arose early during the 2013-2016 epidemic and dominated the viral population. Two studies by Diehl et al. and Urbanowicz et al. now reveal that this mutation is associated with higher infectivity to human cells, representing the clearest example of Ebola's functional adaptation to human hosts.

One of the many mutations that have occurred in viral genome is V483A mutation, which a part receptor-binding motif present S1 domain spike protein. mutant virus popular North America with 36 cases so far and frequently occurring recent days. This review compares wild-type mutants to analyze certain factors like interaction between host-cell interface, binding affinity, stability, partition ene...