A seed lot of strain SF 1684 of rhinovirus type 2 prepared in human embryonic lung cells (WI-38) contained aggregates which interfered with its neutralization by homotypic or homologous antisera. The same virus showed no evidence of aggregation at five other passage levels studied. Virus in the seed lot was not identified correctly, and the titer of homologous antiserum was mistakenly considere...

BACKGROUND A vaccine is needed to control the spread of human immunodeficiency virus type 1 (HIV-1). An in vitro assay that can predict the protection induced by a vaccine would facilitate the development of such a vaccine. A potential candidate would be an assay to quantify neutralization of HIV-1. METHODS AND FINDINGS We have used sera from rhesus macaques that have been immunized with HIV ...

Soluble antigen prepared from pseudorabies virus-infected embryonic pig kidney cells was used to demonstrate the presence of pseudorabies virus antibodies in sera taken from naturally and experimentally infected swine. Antibody could be detected by agar-gel immunodiffusion as early as 14 days postexposure and was demonstrable at least 3 months after experimental infection (the longest period te...

This study was aimed at evaluating the changes in antibody titers and neutralization ability before after inoculation with an mRNA COVID-19 vaccine (Pfizer, USA) among healthcare professionals (HCPs) Japan. A total of 221 HCPs were enrolled their blood samples collected three time points: 1) vaccination, 2) 3 weeks first dose, 3) second dose. The anti-SARS-CoV-2 Spike protein IgG measured all u...

abstract background and aims: to identify and isolation of viruses a susceptible cell is need which can be primary or line. the use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. irkhbk cell line was originated from primary cells of bovine kidney by continuous passage. the aim of this study was to introduce the irkhbk cell lin...

Hantaviruses cause hemorrhagic fever with renal syndrome in Europe and Asia. There are about 20 documented hantavirus species and newer species are being described worldwide, especially in non-rodent reservoirs, i.e shrews. Focus reduction neutralization test is the classical serotyping technique for hantavirus. However, this study employs a previously established serotyping ELISA, to retrospec...

Using the microtiter system, titration of Newcastle disease virus infectivity and neutralizing antibodies was carried out in chicken embryo fibroblasts grown in "U" or flat-bottomed plates. Infectivity was detected by a combined hemadsorption-hemagglutination method. Inhibition of that reaction indicated the presence of neutralizing antibodies. A 24-h microneutralization test was developed and ...

of the Joint International Tropical Medicine Meeting (JITMM). Bangkok, Thailand. 29 November-1 December 2004:106. EVALUATION OF A SINGLE-DILUTION PLAQUE REDUCTION NEUTRALIZATION TEST (PRNT) AS A DIAGNOSTIC TOOL FOR DETECTING INTERCURRENT DENGUE VIRUS (DENV) INFECTIONS Thomas SJ, Nisalak A, Halstead S, Endy T, Lutthiwongsakorn N, Poolpanichupatam Y, Sawatwong P, Changnak S and Mammen MP Jr Studi...

Glycoprotein B (gB) of human cytomegalovirus (HCMV) is the dominating protein in the envelope of this virus and gives rise to virus-neutralizing antibodies in most infected individuals. We have previously isolated a neutralizing human antibody specific for antigenic domain 2 (AD-2) on gB, a poorly immunogenic epitope, which nevertheless is capable of eliciting potent neutralizing antibodies. In...