BACKGROUND & OBJECTIVES There is no published literature on the extent of vitamin B₁₂ deficiency in elderly Indians as determined by plasma vitamin B₁₂ levels and methylmalonic acid (MMA) levels. Vitamin B₁₂ deficiency is expected to be higher in elderly Indians due to vegetarianism, varied socio-economic strata and high prevalence of Helicobacter pylori infection. We therefore, studied the die...

A roundtable to discuss the measurement of vitamin B-12 (cobalamin) status biomarkers in NHANES took place in July 2010. NHANES stopped measuring vitamin B-12-related biomarkers after 2006. The roundtable reviewed 3 biomarkers of vitamin B-12 status used in past NHANES--serum vitamin B-12, methylmalonic acid (MMA), and total homocysteine (tHcy)--and discussed the potential utility of measuring ...

BACKGROUND We evaluated whether measurement of vitamin B(12)-saturated transcobalamin (holo-TC) concentrations or TC saturation (holo-TC:total TC) reflects active vitamin B(12) absorption in healthy individuals and patients after vitamin B(12) intake. METHODS We obtained blood samples from 31 healthy individuals (age range, 25-57 years) before (days -1 and 0) and after (days 1, 2, and 6) oral...

The effect of 10% carbon dioxide on the sensitivity to four antibiotics of 10 strains of Bacteroides fragilis was studied. The minimum inhibitory concentrations of erythromycin and lincomycin hydrochloride for these strains were four to 32 times higher, when grown in hydrogen plus 10% carbon dioxide, than the values obtained when the strains were grown in pure hydrogen. A similar effect was obt...

Vitamin B(12) is essential for DNA synthesis and for cellular energy production.This review aims to outline the metabolism of vitamin B(12), and to evaluate the causes and consequences of sub-clinical vitamin B(12) deficiency. Vitamin B(12) deficiency is common, mainly due to limited dietary intake of animal foods or malabsorption of the vitamin. Vegetarians are at risk of vitamin B(12) deficie...

MANY types of micro-organisms have been tested by various workers for their ability to synthesize "vitamin B" (=complex). In such studies the organisms were grown in various nutrient media and the " vitamin B " was detected by its growth-promoting action or by curative tests on experimental animals such as the rat, the mouse or the pigeon. Thus "vitamin B " has been reported to have been produc...

In order to identify the effects of vitamin B 12 and folic acid in reducing methanolinduced retinopathy, ERG waves were compared before and after methanol intoxication in rabbits. In this study 34 rabbits were divided into 4 groups. All groups received 4 g/kg of methanol intraperitoneally. In addition, the second, third and fourth groups received vitamin B 12 injection, oral folic acid and ...

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical dual wavelength spectrophotometric method for the simultaneous determination of Ambroxol Hydrochloride and Desloratadine Hydrochloride in combined tablet dosage form. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional ...

Orthopaedic surgery has one of the most painful post-operative periods. Pain management is an important consideration in department. The purpose this study was to assess effect Nefopam hydrochloride and Tramadol postoperative analgesia patients undergoing long bone fracture fixations. Triple blindedRandomization allocation groups were carried out by odd even number method. conducted tertiary ca...

The high prevalence of vitamin B-12 deficiency in many regions of the world is becoming recognized as a widespread public health problem, but it is not known to what extent this deficiency results from a low intake of the vitamin or from its malabsorption from food. In rural Kenya, where a previous study identified a high prevalence of inadequate vitamin B-12 intakes, this study examined whethe...