OBJECTIVES Total joint arthroplasty is one of the most frequent and effective surgeries today. However, despite improved surgical techniques, a significant number of implant-associated infections still occur. Suitable in vitro models are needed to test potential approaches to prevent infection. In the present study, we aimed to establish an in vitro co-culture setup of human primary osteoblasts...

titanium (ti) is one of the most widely used biomaterials for manufacturing dental implants. the implant surface properties strongly influence osseointegration. the aim of the present study was to in vitro investigate the characteristics of ti dental implants in terms of mutagenicity, hemocompatibility, biocompatibility, osteoinductivity and biological safety. the ames test was used to test the...

An injectable hydrogel composite consisting of oligo(poly(ethylene glycol)fumarate) (OPF) and gelatin microparticles has been developed as a novel carrier system for cells and growth factors. Rabbit marrow mesenchymal stem cells (MSCs) and gelatin microparticles (MPs) loaded with insulin-like growth factor-1 (IGF-1), transforming growth factor-beta1 (TGF-beta1), or a combination of both growth ...

background: fibroblasts are one of the important cells in wound healing. these cells create a proper bed for keratinocytes migration and wound contraction.wound healing in distal limb of horses has complications, such as formation of exuberant granulation tissue (egt). the main factor in this problem is overgrowing of fibroblasts. objectives: the purpose of the present study was to compare fibr...

Purpose: In this study, it is aimed to prepare chitosan nanoparticles containing shRNA-VEGF and evaluate their bioactivity by in vitro cell culture studies perform mechanical characterization of nanoparticles.
 Material Methods: Ionic chelation method was used nanoparticles. The XTT assay assess the cytotoxic activity loaded NP on HeLa NIH 3T3 cells.
 Results: According results IC50 v...

developing supernumerary limbs is a rare congenital condition that only a few cases have been documented. depending on the cause and developmental conditions, they may be single, multiple or complicated, and occur as a syndrome or associated with other anomalies. polymelia is defined as the presence of extra limb(s) which have been reported in human, mouse, chicken, calf and lamb. it seems that...

.........................................................................................................................2 INTRODUCTION ................................................................................................................6 CHAPTER 1 CHARACTERISTICS OF NOVEL MICRONEEDLE ARRAYS FABRICATED FROM HYALURONIC ACID ................................................................

Nacrtak Forest biomass from timber harvesting residues is often used during mechanized forest opera­ tions to improve trafficability of strip roads (machine operating trails). In particular, during cut-to-length operations brush mats from harvesting residues are created on operating trails to reduce rutting. However, forest biomass is becoming increasingly important as a source of renewable ene...

Co-culture models are currently bridging the gap between classical cultures and in vivo animal models. Exploring this novel approach unlocks the possibility to mimic the tumor microenvironment in vitro, through the establishment of cancer-stroma synergistic interactions. Notably, these organotypic models offer a perfect platform for the development and pre-clinical evaluation of candidate nanoc...

OBJECTIVE To prepare and evaluate the liver-targeted drug delivery system of Bufalin with higher liver uptake and stronger antitumor activity against hepatocellular carcinoma. METHODS Bufalin-loaded bovine serum albumin nanoparticle was prepared by desolvation method, to investigate the in vitro release performance and to evaluate the pharmacokinetic and tissue distribution. The antitumor act...