Ever since the inception of STR-based forensic DNA analysis for human identification and profiling, approach developed continuously. Most these developments are pertaining to achieve set best polymorphic loci analysis, high inhibitor tolerance capability, increase in sensitivity, reduction turnaround time, cost-effectiveness. On similar line, SF25™ PCR amplification kit, a 6 fluorescent dye-che...

The analysis of polymorphic microsatellites is relevant for forensic, paternity testing and to study genetic admixtures and the genetic relationships between populations. Colombia has the second highest number, after Brazil, of Amerindian tribes in America (around 81) followed by Mexico (68 tribes) and Venezuela (20 tribes). Contrary to other genetic markers such as HLA and blood groups, Short ...

Monozygotic twins can be co-identified by genotyping of short tandem repeats (STRs); however, for distinguishing them, STR genotyping is ineffective, especially in the case of murder. Here, a rarely occurring tri-allelic pattern in the vWA locus (16, 18, 19) was identified only in the DNA of one identical twin, which could help to exonerate the innocent twin in a murder charge. This mutation wa...

ANTXR 1 and 2, also known as TEM8 and CMG2, are two type I membrane proteins, which have been extensively studied for their role as anthrax toxin receptors, but with a still elusive physiological function. Here we have analyzed the importance of N-glycosylation on folding, trafficking and ligand binding of these closely related proteins. We find that TEM8 has a stringent dependence on N-glycosy...

Pernambuco is one of the 27 federal units of Brazil, ranking seventh in the number of inhabitants. We examined the allele frequencies of 13 short tandem repeat loci (CFS1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, vWA, and TPOX), the minimum recommended by the Federal Bureau of Investigation and commonly used in forensic genetics laboratories in Brazil, in...

Rapid, cost-effective methods for high-throughput DNA analysis are needed to process samples currently being gathered for large criminal DNA databases around the world. Within the U.S., several states have sample backlogs of over 50,000 samples with limited funds and manpower to analyze these samples. Currently available slab gel or capillary electrophoresis instruments can handle only a few do...