The ion channels located on the cell fine structures play an important role in the physiological functions of cell membrane. However, it is impossible to achieve precise positioning on the nanometer scale cellular microstructures by conventional patch-clamp technique, due to the 200 nm resolution limit of optical microscope. To solve this problem, we have established a high-resolution patch-cla...

The recent development of automated patch clamp technology has increased the throughput of electrophysiology but at the expense of visual access to the cells being studied. To improve visualization and the control of cell position, we have developed a simple alternative patch clamp technique based on microfluidic junctions between a main chamber and lateral recording capillaries, all fabricated...

The membrane clamp technique is an important tool to reflect the electrophysiological characteristics of cells by recording ionic currents cellular channels. Embedded systems work in designed for specific user groups and are used implement functions. amplifier built with embedded technology has advantages miniaturization, specialization, low power consumption, high integration, resource utiliza...

29 THE 4TH ANNUAL IEEE INTERnational Conference on Nano/Microengineered Molecular Systems (IEEE-NEMS) was held at the Sheraton Dameisha Resort in Shenzhen, China, 5–8 January 2009. More than 300 papers were presented by leading micro/nanotechnology researchers in academia and industry from around the globe. Topical areas of the conference included microelectro mechanical systems (MEMS), BioMEMS...

Extracellular ATP affects a wide variety of cells via purinergic membrane receptors. One class of purinergic receptors, P2X, consists of ATP-gated, calcium-permeable, cation-selective channels. We performed whole cell patch-clamp studies, intracellular calcium concentration ([Ca2+]i) measurements, and reverse transcription-polymerase chain reaction (RT-PCR) to determine whether P2X receptors ar...

UNLABELLED Whole-cell patch-clamp recordings in rat cerebellar slices were used to investigate the effect of metabotropic glutamate receptor activation on mossy fibre-granule cell synaptic transmission. Transient application of 20 microM 1S, 3R-aminocyclopentane-1, 3-dicarboxylic acid simultaneously with low-frequency NMDA receptor activation induced long-lasting non-decremental potentiation of...