Background Dystrophin, an essential protein functional in the maintenance of muscle structural integrity is known to be responsible for muscle deterioration during white spot syndrome virus (WSSV) infection among prawn species. Previous studies have shown the upregulation of dystrophin protein in Macrobrachium rosenbergii (the giant freshwater prawn) upon white spot syndrome virus (WSSV) infect...

A portion of the VP28 gene (VP28F118) of white spot syndrome virus was cloned into an expression vector and transformed into E. coli. The objective was to produce a truncated VP28 envelope protein lacking the N-terminal transmembrane region. After induction, the recombinant VP28F118 protein (rVP28F118) was produced, purified by SDS-PAGE and used to immunize Swiss mice for monoclonal antibody pr...

Currently the most common and devastating disease of shrimp is caused by the white spot syndrome virus (WSSV), which has spread throughout the world mainly by different species of crustaceans carrying the virus. Penaeus monodon were challenged with WSSV and the protein expression variation studied over a period of time. The gills, one of the main target organs of WSSV, showed upregulation of 24...

El aceite de coco (Cocos nucifera) es considerado una sustancia inmunoestimulante natural que contiene compuestos bioactivos con la capacidad regular o modificar respuesta inmune e incrementar resistencia contra agentes patógenos. Es por ello el objetivo esta investigación fue evaluar C. nucifera suplementado en dieta como potencial antiviral para reducir las mortalidades ocasionadas virus manc...

The present study investigated the in vivo hemocytic and hepatopancreatic response to Vibrio parahaemolyticus and white spot syndrome virus (WSSV) injection in shrimp Litopenaeus vannamei. The proliferation of bacteria and virus in shrimp, animal mortality, total hemocyte counts (THCs), phenoloxidase (PO) activity, respiratory burst, and gene expression of immune factors associated with immune ...

OBJECTIVE To determine whether viable White Spot Syndrome virus (WSSV) or Yellowhead virus (YHV) were present in prawn products imported into Australia. PROCEDURE A sample of fourteen uncooked prawns was obtained from a consignment imported from southeast Asia. Each of the prawns was examined for WSSV by polymerase chain reaction (PCR), and then a bioassay was conducted in which a 10% homogen...

Red swamp crayfish Procambarus clarkii is used for the innate immune defense of crustaceans due to its convenience for laboratory culture and study. To know more about the transcriptome of the crayfish, we constructed and sequenced a cDNA library from a mixture of hemocytes and hepatopancreas from white spot syndrome virus (WSSV)-infected crayfish. By random sequencing, we obtained 9115 high-qu...

In shrimp, higher water temperatures (~32°C) can suppress the ability of white spot syndrome virus (WSSV) to replicate and cause mortality, but the mechanisms remain unclear. To investigate whether cell apoptosis might be involved, a Tdt-mediated dUTP nick-end label (TUNEL) method was used to assess levels of chromosomal DNA fragmentation in hepatopancreas and gill cells of Procambarus clarkii ...

Previous studies have discovered a lot of immune-related genes responding to white spot syndrome virus (WSSV) infection in crustacean. However, little information is available in relation to underlying mechanisms of host responses during the WSSV acute infection stage in naturally infected shrimp. In this study, we employed next-generation sequencing and bioinformatic techniques to observe the ...

The objective of the present investigation was to determine the level of WSSV transmission from the infected tiger prawn Penaeus monodon to SPF Litopenaeus vannamei in laboratory captivity condition, in connection with to PCR detection and viral genome sequence. The nested PCR product band was ranged between 296 to 910 bp and was found during entire period from the experimental studies of SPF L...