Sterylglucosides (SGs) are composed of a glucose and sterol derivatives, and are distributed in fungi, plants and mammals. We recently identified EGCrP1 and EGCrP2 (endoglycoceramidase-related proteins 1 and 2) as a β-glucocerebrosidase and steryl-β-glucosidase, respectively, in Cryptococcus neoformans. We herein describe an EGCrP2 homologue (Egh1; ORF name, Yir007w) involved in SG catabolism i...

In order to investigate the effects of water stress on total phenolics content, antioxidant power, β-glucosidase activity and stomatal properties of alfalfa, a factorial experiment based on randomized complete block design was carried out in 1-Lit pots containing half strength Hoagland culture medium using two cultivars of alfalfa at four osmotic pressures including 0 (control), -0.5, -1.0 and ...

Cheonggukjang, a traditional soy food, was prepared with addition of Lentinus edodes powder at 0, 5, and 10% (w/w), and the distribution of isoflavones were analyzed by high performance liquid chromatography during fermentation for 48 h. β-Glucosidase activity and changes in succinyl genistin and succinyl daidzin were monitored. β-Glucosidase activity in cheonggukjang with 5 and 10% (w/w) L. ed...

Genomic and transcriptomic approaches were used to gain insights into the relationship between soil management and bacterial-mediated functions in an olive orchard agroecosystem. Four management practices were assessed in a 30-year trial in a semiarid Mediterranean region. Transcriptional activity of bacterial 16S rRNA genes increased in noncovered soils, indicating higher microbial maintenance...

The economic conversion of lignocellulosic biomass to biofuels requires in addition to pretreatment techniques access to large quantities of inexpensive cellulases to be competitive with established first generation processes. A solution to this problem could be achieved by plant based expression of these enzymes. We expressed the complete set of six cellulases and an additional β-glucosidase e...

For the beneficial pharmacological properties of resveratrol, there is increasingly interest in enzymatic conversion of polydatin to resveratrol. The metagenomic technique provides an effective strategy for mining novel polydatin-hydrolysis enzymes from uncultured microorganisms. In this study, a metagenomic library of mangrove soil was constructed and a novel β-glucosidase gene MlBgl was isola...

The floating island system exploits the combination of aquatic plants, microorganisms, and extracellular enzymes to purify wastewater. We investigated purification efficiency eight plant species cultured in relationships capacity with enzyme activity microbial community were analyzed explore crucial factors that affect mechanism pollutants removal different systems. Three species, namely Oenant...

the rose sawfly, arge rosae linnaeus, is one of the most destructive pests of rose bushes in the north of iran. nowadays, many attempts have been made to reduce pesticide application by looking for new methods of pest control. a non chemical method for controlling insect pests including a. rosae can be achieved by using genetically engineered plants expressing carbohydrase inhibitors. therefore...