β-Lactoglobulin (BLG) like other lipocalins can be modified by mutagenesis to re-direct its ligand binding properties. Local site-directed was used change the geometry of BLG pocket and therefore preferences. The presented studies are focused on previously described mutants L39Y, I56F, L58F, F105L, M107L two new variants, L39K F105A, their interactions with local anesthetic drug tetracaine. Bin...

Lactoferrin (LF) and β-lactoglobulin (BLG), strongly basic and weakly acidic bovine milk proteins, form optically clear coacervates under highly limited conditions of pH, ionic strength I, total protein concentration C(P), and BLG:LF stoichiometry. At 1:1 weight ratio, the coacervate composition has the same stoichiometry as its supernatant, which along with DLS measurements is consistent with ...

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A wide variety of crystallization solutions are screened to establish conditions that promote the growth of a diffraction-quality crystal. Screening these conditions requires the assessment of many crystallization plates for the presence of crystals. Automated systems for screening and imaging are very expensive. A simple approach to imaging trace fluorescently labeled protein crystals in cryst...

Background When exclusive breastfeeding is not possible, partially hydrolyzed formula (PHF) often used as a starter for infants. Some children develop allergic symptoms, including anaphylaxis, after the first intake of cow protein. Therefore, tolerability PHF in infants with cow's milk allergy (CMA) important information. Partially whey (PHWF) well characterized, but those containing both and c...

BACKGROUND Drug nanosuspensions are very promising for enhancing the dissolution and bioavailability of drugs that are poorly soluble in water. However, the poor stability of nanosuspensions, reflected in particle growth, aggregation/agglomeration, and change in crystallinity state greatly limits their applications. Solidification of nanosuspensions is an ideal strategy for addressing this prob...

BACKGROUND Cow's milk allergy diagnosis many times requires double-blind placebo-controlled food challenge (DBPCFC), which presents high accuracy but involves risks, specifically in infants and anaphylactic patients. The identification of the cut-off values for specific IgE to milk or its components would contribute to cow's milk allergy (CMA) diagnosis. The aim of this study was to compare dis...