The effect of zinc ions (Zn(I1)) on the activation of factor XI1 in the absence of a procoagulant surface was investigated by initial velocity kinetic studies at I = 0.16, pH 7.4, and 26 “C. Zinc ions at concentrations greater than 160 p M potentiated 99-fold the kcat/KM for the activation of factor XI1 by kallikrein and, at an optimum concentration of 110 pM, accelerated 140fold the apparent k...

Choline-binding modules are present in some virulence factors and many other proteins of Streptococcus pneumoniae (Pneumococcus). The most extensively studied choline-binding module is C-LytA, the C-terminal moiety of the pneumococcal cell-wall amidase LytA. The three-dimensional structure of C-LytA is built up from six loop-hairpin structures forming a left-handed beta-solenoid with four choli...

The nuclear modulation effect in pulsed EPR spectroscopy was used to probe the metal-protein bonds in Cu(II)=transferrin complexes as a function of pH. In preparations in equilibrium with air at pH 4.8 no modulation features other than those attributable to protons were observed, in keeping with conventional EPR studies indicating that at this pH only nonspecific binding takes place (Zweier, J....

Mn(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II), and Hg(I1) form metallocarbonic anhydrases containing these metal ions at the same binding site. Of this series, only the Zn(I1) and Co(I1) enzymes catalyze the hydration of CO2 and the hydrolysis of p-nitrophenyl acetate. Likewise, only Zn(I1) and Co(I1) induce the metal ion-dependent binding of 3H-acetazolamide at the active center. Acetate, azi...

The reaction between hydrogen peroxide and ferrous EDTA generates an oxidizing intermediate (I1) which is not the hydroxyl radical. It oxidizes ferrocytochrome c and also reacts with hydrogen peroxide (k5 = 3.2 X 10(3) M-1 S-1) to form a second oxidizing transient (I2). I1 is not scavenged by t-butyl alcohol whereas I2 is. I1 is found to be significantly less reactive than the hydroxyl radical ...

Bovine trophoblast protein-1 (bTP-1) is a Type I interferon secreted by the bovine trophoblast from about Day 15 of pregnancy. It is not known whether bTP-1 has functional properties in common with other interferons. The aim of the present study was to determine whether bTP-1 inhibits proliferation of lymphocytes induced by mitogens, mixed lymphocyte cultures (MLC) and interleukin-2 (IL-2) and,...

Mathematical modelling provides a comprehensive understanding of the charge transport process inside solar cells and determines various elements affecting their performance. In present paper, proposed CH3NH3Pb(I1−xBrx)3-based perovskite consisting Cd1−xZnxS as electron layer (ETL) have been mathematically simulated using SCAPS-1D simulator. Hole layers (HTLs) poly(3-hexylthiophene) (P3HT), Cu2O...

the synthesis and single-crystal x-ray structure determination of the title compound (dinitrato) (1,4,8,11 -tetra-azaandecane) lead(ii) is described. crystals are monoclinic, p2 /ca= 10.271 (2)?,b= 13.524(4) ?,c= 11.298(6) ?,?=94.68(3),2=4,r,rw, 0.036 and 0.041, respectively. the lead atom is eight-coordinate [pb-n, 2.44 (2) - 2.55 (1)], [pb-0,2.87 (1) - 3.06 (2)].