صخلملا A total of 48 fresh water fishes, belonging to four species of the family Cyprinidae (Capoeta trutta, Chalcalburnus mossulensis, Cyprinion macrostomum and Leuciscus lepidus) were collected from Darbandikhan lake, southwest Sulaimaniya city, Kurdistan region, in the north of Iraq, from March to the end of July 2008. The inspection of gills revealed the infection of these fishes with four ...

Unlike population-level approaches, single-cell RNA sequencing enables transcriptomic analysis of an individual cell. Through the combination of high-throughput sequencing and bioinformatic tools, single-cell RNA-seq can detect more than 10,000 transcripts in one cell to distinguish cell subsets and dynamic cellular changes. After several years' development, single-cell RNA-seq can now achieve ...

ش ي ر ي ن ي فسو ي نا 1 (M.Sc) ، س ي د ما ي د انعر يي س ي تدا 1 (Ph.D) ، ونهد ناسحا ي 1 و 2 (Ph.D) ، قتدمحم ي جرب ي نا ـنجورب ي 1 (M.Sc) زاـنرف ن ي دازك نانمج ي 1 و 3 (M.Sc) 1 هاگشناد هش ي د تشهب ي ، هدكشناد ي سدنهم ي ژرنا ي روانف و ي اه ي ون ي ،ن امزآ ي هاگش بونان ي ژولونكتو ي ، ب هورگ ي ژولونكتو ي 2 هاگشناد برت ي ت هدكشناد ،سردم ي ز مولع ي تس ي 3 هاگشناد ملا...

salinization of crop lands is one of the most important reasons for reduction in productivity and threats food security. activation of signaling process in plants is the first step during sense environmental cues and stress signals. identification of the components of plant intracellular signaling mechanisms, pathways, and networks in response to salt stress in aegilops tauschii (the d genome d...

In eukaryotic cells, alternative cleavage of 3' untranslated regions (UTRs) can affect transcript stability, transport and translation. For polyadenylated (poly(A)) transcripts, cleavage sites can be characterized with short-read sequencing using specialized library construction methods. However, for large-scale cohort studies as well as for clinical sequencing applications, it is desirable to ...

C57BL/6J (B6) and DBA/2J (D2) are two of the most commonly used inbred mouse strains in neuroscience research. However, the only currently available mouse genome is based entirely on the B6 strain sequence. Subsequently, oligonucleotide microarray probes are based solely on this B6 reference sequence, making their application for gene expression profiling comparisons across mouse strains dubiou...

The continuously prolonged human lifespan is accompanied by increase in neurodegenerative diseases incidence, calling for the development of inexpensive blood-based diagnostics. Analyzing blood cell transcripts by RNA-Seq is a robust means to identify novel biomarkers that rapidly becomes a commonplace. However, there is lack of tools to discover novel exons, junctions and splicing events and t...

Signaling via B cell receptors (BCR) and Toll-like receptors (TLRs) result in activation of B cells with distinct physiological outcomes, but transcriptional regulatory mechanisms that drive activation and distinguish these pathways remain unknown. At early time points after BCR and TLR ligand exposure, 0.5 and 2 h, RNA-seq was performed allowing observations on rapid transcriptional changes. A...

RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene regulation, and how these aspects depend on each other. Here, I review the current single cell RNA-seq (scRNA-seq) efforts and discuss experim...