Transcription factor (TF) IIIB, the central transcription initiation factor of RNA polymerase III (pol III), is composed of three subunits, Bdp1, Brf1 and TATA-binding protein (TBP), all essential for normal function in vivo and in vitro. Brf1 is a modular protein: Its N-proximal half is related to TFIIB and binds similarly to the C-terminal stirrup of TBP; its C-proximal one-third provides mos...

The TATA-binding protein (TBP) is critical for transcription by all three nuclear RNA polymerases. In order to identify factors that interact with TBP, the nonnatural photoreactive amino acid rho-benzoyl-phenylalanine (BPA) was substituted onto the surface of Saccharomyces cerevisiae TBP in vivo. Cross-linking of these TBP derivatives in isolated transcription preinitiation complexes or in livi...

کانسار فسفات دلیر در 57 کلیومتری جنوب غربی شهر چالوس استان مازندران و زون البرز مرکزی دارد. میزبان این ذخیره سازند سلطانیه است که یکی از مهم‌ترین سازندهای رسوبی ایران است. نتایج آنالیز پراش اشعه ایکس (XRD) مطالعات میکروسکوپ نوری الکترونی بر روی نمونه‌های نشان داد آپاتیت، کلسیت، کوارتز دولومیت کانی‌های اصلی فرعی شامل مونتموریلونیت، پیریت باریت مقادیر به همراه جزیی سیدریت، روتیل، ایلیت گوتیت هستن...

Platinum group metals (PGMs) are important for the manufacture of advanced materials in field catalysts and electronic devices. Since chemical properties PGMs very similar to each other, hydrometallurgical processes should be employed recover with high purity from either ores or secondary resources. In PGMs, first step is dissolution PGMs. For this purpose, inorganic acid solutions oxidizing ag...

TFIIB is an RNA polymerase II general transcription factor (GTF) that has also been implicated in the mechanism of action of certain promoter-specific activators (see, for examples, [1-11]). TFIIB enters the preinitiation complex (PIC) primarily through contact with the TATA box binding protein (TBP), an interaction mediated by three TBP residues [12-14]. To study the role of TFIIB in transcrip...

The multi-protein complex SL1, containing TBP, which is essential for RNA polymerase I catalyzed transcription, has been analyzed in fission yeast. It was immunopurified based on association of component subunits with epitope-tagged TBP. To enable this analysis, a strain of Schizosaccharomyces pombe was created where the only functional TBP coding sequences were those of FLAG-TBP. RNA polymeras...

Binding of the TATA-binding protein (TBP) to the promoter is the first and rate limiting step in the formation of transcriptional complexes. We show here that nuclear import of TBP is mediated by a new karyopherin (Kap) (importin) family member, Kap114p. Kap114p is localized to the cytoplasm and nucleus. A complex of Kap114p and TBP was detected in the cytosol and could be reconstituted using r...

Oxidative stress plays an essential role in the development of age-related cataract. Thioredoxin binding protein-2 (TBP-2) is a negative regulator of thioredoxin (Trx), which deteriorates cellular antioxidant system. Our study focused on the autophagy-regulating effect of TBP-2 under oxidative stress in human lens epithelial cells (LECs). Human lens epithelial cells were used for cell culture a...

A single gene encodes the TATA binding protein (TBP) in yeasts and animals. Although two TBP-encoding genes (Tbp) previously were isolated from both Arabidopsis and maize, the expression and in vivo function of the encoded plant TBPs were not investigated. Here, we report that the two highly conserved maize Tbp genes are unlinked and reside within larger, ancestrally duplicated segments in the ...