background: species of microsporidia have been known as opportunistic obli-gate intracellular parasites particularly in immunocompromised patients. enterocyto-zoon bieneusi is one of most prevalent intestinal microsporida parasites in hiv + /aids patients. in this study, intestinal microsporidia infection was deter-mined in hiv + /aids patients using microscopic and molecular methods. methods: ...

background: the objective of the study was to evaluate the presence of neospora caninum organisms in the brain of aborted fetuses and placentas of full-term calves born of seropositive cows. methods: during 2006-2007, 12 brains of aborted calves from neospora seropositive cattle and 7 pla­centas from seropositive dams giving birth to full-term calves, from four dairy cattle farms located around...

objective :cryptosporidiosis is one of the most important parasitic infections in iran which causes diarrhea in humans and animals. the identification of the cryptosporidium species among humans is necessary. this study aims to identify species of cryptosporidium isolated from patients that referred to three hospitals in tehran based on the 18s rrna gene by nested pcr-rflp assay. ‎methods :in t...

A colorimetric one-tube nested PCR was developed for the detection of Trichomonas vaginalis in clinical vaginal discharge specimens. A family of 650-bp specific DNA repeats from the T. vaginalis genome was targeted. There was no cross-reaction with human DNA or other infectious agents, including Pentatrichomonas hominis and Giardia lamblia. The colorimetric assay was applied as an adjunct to ne...

Problem statement: Q fever is a ubiquitous zoonosis caused by Coxiella burnetii, an obligate intracellular rickettsial organism that caused abortion and stillbirth in ruminants. Approach: The prevalence of Coxiella burnetii in Iran is essentially unknown. Its traditional diagnosis is based on culture, serology and conventional PCR. In this present study, for more sensitive and accurate detectio...

For the rapid identification and detection of Vibrio anguillarum, we have PCR amplification technique targeting gyrB region has been evaluated. We have designed twosets of PCR primers for the specific amplification of gyrB in V. anguillarum using single and nested PCR. PCRs specificity was demonstrated by successful amplicon from V. anguillarum DNA. The detection limit of the single PCRs and th...

A nested reverse transcriptase (RT) PCR assay detected mRNA of the salmonid pathogen Renibacterium salmoninarum in samples of RNA extracts of between 1 and 10 cells. Total RNA was extracted from cultured bacteria, Atlantic salmon (Salmo salar L.) kidney tissue and ovarian fluid seeded with the pathogen, and kidney tissue from both experimentally challenged and commercially raised fish. Followin...