Tumor necrosis factor (TNF) signals cell death and simultaneously induces generation of ceramide. To evaluate the contribution of ceramide to TNF-dependent cell death, we generated clones of the TNF-sensitive cell line L929 that constitutively overexpress human acid ceramidase (AC). Ceramidase, in concert with sphingosine kinase, metabolizes ceramide to sphingosine-1-phosphate (SPP), an inducer...

We have previously shown that lactoperoxidase (LPO) covalently coupled to polystyrene tissue culture flasks can be used to radioiodinate monolayer cell proteins that come into intimate contact with the LPO-polystyrene surface. These studies have now been extended to include a detailed examination of the class of iodinated polypeptides migrating with apparent molecular weights of 50,000 and 55,0...

This study aimed to evaluate the cytotoxicity of a crude extract of Piper cubeba against normal and breast cancer cell lines. To prepare the extract, P. cubeba seeds were ground, soaked in methanol and dichloromethane and isolated by column chromatography. Fractions were tested for cytotoxicity effects on normal fibroblast (L929), normal breast (MCF-12A) and breast cancer cell lines (MCF-7, MDA...

OBJECTIVE The aim of this study was to investigate the cytotoxicity of two different bioactive root canal sealers: one based on mineral trioxide aggregate, MTA Fillapex (Angelus, Solucoes Odontologicas, Londrina, PR, Brazil), and the other based on bioceramics, Endosequence BC Sealer (Brasseler, Savannah, Georgia, USA), in culture of mouse L929 fibroblasts. MATERIALS AND METHODS Mouse fibrobl...

Tumor necrosis factor (TNF) kills some types of tumor cells in vitro and participates in tumor elimination in vivo. TNF has been shown to kill cells by altering their mitochondria structurally and functionally. The oncogene BCL-2 codes for a protein located in the inner membrane of mitochondria which is able to inhibit the commitment to cell death in various cell types. We have therefore invest...

در این پژوهش تقویت باریکه لیزر تپی فمتوثانیه Ti:sapphire ­کننده بازتولیدی با آرایش هندسی Z براساس روش تپ چیرپ بررسی شده است. برای انتقال ورودی اولیه به داخل کاواک و استخراج از دو سلول پاکل استفاده زمان شکل‌گیری برحسب انرژی دمش مطالعه روند تحول مورد قرار گرفته مدت تولید بدون حضور 80 نانوثانیه فرایند 38 نرخ تکرار 10 هرتز طول موج مرکزی 800 نانومتر بیشینه 2 میلی‌ژول بعد 17 رفت برگشت 15 میلی ژول 532...

Cocaine has been demonstrated to have a number of different effects on immune cell functions. We have reported alterations of cellular functions by macrophages (Mphi) exposed to cocaine in vitro, including the inhibition of mouse hepatitis virus replication. Here, we present evidence that cocaine stimulates the secretion of an antiviral product that is neutralized by anti-interferon (anti-IFN)....

The main objective of the present investigation is to evaluate the phytochemical constituents, cytotoxic and antiproliferative effect of Karumsurathi Thailam prescribed by the Traditional Siddha Practitioner of Kanyakumari District, India. The external form of Breast Cancer medicine prescribed above 18 years was prepared with 18 different plant ingredients. Phytochemical analysis of the Thailam...

Titanium dioxide nanoparticles (TiO₂ NPs) are widely used in sunscreens, cosmetics and body implants, and this raises toxicity concerns. Although a large number of reports claim that they are safe to use, others claim that they induce reactive oxygen species formation and can be carcinogenic. In this study, the origins of toxic response to TiO₂ NPs were investigated by using Surface-enhanced Ra...