We compared the efficiency of transformation of recombinant M13 DNA obtained using the traditional calcium chloride method with that obtained by electroporatlon. M13 cloning experiments are often hampered by low yields I.e. insufficient numbers of recombinant plaques are generated. Low concentration of the target DNA, poor ligation due to target DNA impurity and small target fragment size are c...

a. School of Chemistry, University of Nottingham, NG7 2RD Nottingham, UK b. Laboratorio de Microscopías Avanzadas (LMA)—Instituto de Nanociencia de Aragón (INA), Universidad de Zaragoza, 50018 Zaragoza, Spain c. Instituto de Ciencia de Materiales de Aragón, Universidad de Zaragoza-CSIC, 50009 Zaragoza, Spain d. EPSRC National UK EPR Facility, Photon Science Institute, University of Manchester, ...

The deoxytetradecamer d(AAAACGACGGCCAG) has been shown to be an excellent universal primer for sequence determination of DNA cloned into the bacteriophage M13 mp7, mp8, and mp9 series. This new primer offers several advantages over others currently available and it has been used to define the cloning of Hinf I fragments of bacteriophage S13 DNA into the Eco RI site of M13 mp7, utilizing the hom...

John E. Proctor,1,* Eugene Gregoryanz,1 Konstantin S. Novoselov,2 Mustafa Lotya,3 Jonathan N. Coleman,3 and Matthew P. Halsall4 1School of Physics and Centre for Science at Extreme Conditions, University of Edinburgh, Edinburgh EH9 3JZ, United Kingdom 2School of Physics and Astronomy, University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom 3School of Physics, Trinity College D...

Raman spectroscopy is used to study low-wave-number (</=20 cm(-1)) acoustic vibrations of the M13 phage. A well-defined Raman line is observed at around 8.5 cm(-1). The experimental results are compared with theoretical calculations based on an elastic continuum model and appropriate Raman selection rules derived from a bond polarizability model. The observed Raman mode is shown to belong to on...