Solid tumors consist of malignant cells and associated stromal components, including fibroblastic cells that contribute to tumor growth and progression. Although tumor fibrosis and aberrant vascularization contribute to the hypoxia often found in advanced tumors, the contribution of hypoxic signaling within tumor-associated fibroblasts to tumorigenesis remains unknown. In this study, we used a ...

Hypoxia is an important microenvironmental factor in the development of renal fibrosis; however, the underlying mechanisms are not well elucidated. Here we show that hypoxia induces Bmi1 mRNA and protein expression in human tubular epithelial cells. We further demonstrate that Bmi1 expression might be directly regulated by hypoxia-inducible factor-1a (HIF-1a) under low oxygen. Moreover, chromat...

Shimoda, Larissa A., Dominador J. Manalo, James S. K. Sham, Gregg L. Semenza, and J. T. Sylvester. Partial HIF-1a deficiency impairs pulmonary arterial myocyte electrophysiological responses to hypoxia. Am J Physiol Lung Cell Mol Physiol 281: L202–L208, 2001.—Chronic hypoxia depolarizes and reduces K current in pulmonary arterial smooth muscle cells (PASMCs). Our laboratory previously demonstra...

Introduction. The relationship of the violation subpopulation composition blood monocytes in ischemic cardiomyopathy (ICMP) with changes monocytopoiesis, as well effect colony-stimulating factor macrophages (M-CSF) and cytokines on differentiation various immunophenotypes bone marrow is great relevance. Aim – to study role mechanisms local distant regulation classical, intermediate, non-classic...

Background: Fibroblast growth factor 2 (FGF2) is a major angiogenic factor involved in angiogenesis and arteriogenesis, however the regulation of its expression during these processes is poorly documented. FGF2 mRNA contains an internal ribosome entry site (IRES), a translational regulator expected to allow mRNA expression during cellular stress. Methodology/Principal Findings: In the present s...

Renal cell carcinoma (RCC) progression resulting from the uncontrolled migration and enhanced angiogenesis is an obstacle to effective therapeutic intervention. Tumor metabolism has distinctive feature called Warburg effect, which enhances the aerobic glycolysis rapidly supplying the energy for migration of tumor. To manipulate this metabolic change characteristic of aggressive tumors, we utili...

Background: It is expected that the expression of key enzymes glycolysis pathway, specially PFK-1, increases tumor cells and so enhances function this pathway. The p53 HIF-1 proteins are regulators PFK-1 LDH enzymes. This study was performed to investigate changes in LDH-A, p53, HIF-1α genes identify metabolic non-small cell lung carcinoma (NSCLC) samples. Methods: A number 30 tumors their adja...

BACKGROUND Sepsis caused by complicated intra-abdominal infection is associated with high mortality. Loss of endothelial barrier integrity, inflammation, and impaired cellular oxygen have been shown to be primary contributors to sepsis. To date, little is known regarding the pathway for the mobilization of endothelial progenitor cells (EPCs) from the bone marrow in sepsis whereas stromal-cell-d...