A new stable pyrrolidine functionalized fullerene derivative, C69H10N2O2, has been synthesized, purified by high performance liquid chromatography, and characterized by MALDI mass spectrometry, ultraviolet-visible spectroscopy, Fourier transform infrared, H and C nuclear magnetic resonance. The magnetic properties of the analogous endohedral species have been studied by both continuous wave (CW...

Applying recent developments in protein purification techniques, a number of lipoxygenase isoenzymes have been isolated in satisfactory quantities for a detailed physical and structural characterization. Four seed isoenzymes from two soybean cultivars have been studied by peptide mapping, free thiol and iron content determinations, and C-terminal analysis as well as by uv-visible absorption and...

Recently, in vivo electron paramagnetic resonance (EPR) spectroscopy and imaging have been widely used to investigate free radical distribution and metabolism in tissues, organs, and whole body of small animals. Endogenous nitric oxide (NO) is an attractive target of this method. In the present study, NO production from a nitrovasodilator, isosorbide dinitrate (ISDN), in live mice was investiga...

Muscle metabolic by-products during exercise, such as K+, lactic acid, ATP, H+, and phosphate, are well established to be involved in the reflex cardiovascular response to static muscle contraction. However, the role of muscle reactive oxygen species (ROS), a metabolic by-product during muscle contraction, in the exercise pressor reflex (EPR) has not been investigated in detail. In the present ...

Effectively Propositional Logic (EPR), also known as the Bernays-Schönfinkel class, allows encoding problems that are propositional in nature, but EPR encodings can be exponentially more succinct than purely propositional logic encodings. We recently developed a DPLL-based decision procedure that builds on top of efficient SAT solving techniques to handle the propositional case efficiently whil...

Background: In light of plans to implement an electronic patient record (EPR), preparations for radical organisational change were recognised as being critical to success. Aim: To determine hospital staff’s experience of and attitudes toward computer use and the EPR. Method: A cross-sectional design using The Computer and EPR Attitude Survey was administered to 878 health service employees in t...

Rapid freeze-quench (RFQ) in combination with electron paramagnetic resonance (EPR) spectroscopy at X-band is a proven technique to trap and characterize paramagnetic intermediates of biochemical reactions. Preparation of suitable samples is still cumbersome, despite many attempts to remedy this problem, and limits the wide applicability of RFQ EPR. We present a method, which improves the colle...

The argument of Einstein, Podolsky and Rosen (EPR), advanced by these authors in support of the thesis that quantummechanics cannot provide a complete description of a physical system, has been receiving close attention in foundational studies and debates on quantum mechanics ever since the famous paper of EPR appeared in 1935 [1]. Not lacking in subtlety, the EPR argument was bound to be misre...

Electron paramagnetic resonance (EPR) spectroscopy is a powerful and widely used technique for studying structure and dynamics of biomolecules under bio-orthogonal conditions. In-cell EPR is an emerging area in this field; however, it is hampered by the reducing environment present in cells, which reduces most nitroxide spin labels to their corresponding diamagnetic N-hydroxyl derivatives. To d...

We present a comparison of experimental and simulated frequency- and field-domain electron paramagnetic resonance (EPR) spectra of integer and half-integer high-spin transition-metal ion complexes. For the simulation of EPR spectra a new tool within the EPR simulation software EasySpin is introduced, which allows for field- and frequency-domain EPR simulations with the same theoretical model an...