Background: Susceptibility test is a useful tool for selecting and modifying appropriate treatment for tuberculosis. Objective: To evaluate a direct colorimetric assay based on 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) for a rapid detection of rifampicin resistance. Methods: Sputum was inoculated directly into 7H9 broth supplemented with PANTA (antibiotic mixture) and o...

Background:  Cell viability is an important factor in radiation therapy and thus is a method to quantify the effect of the therapy. Materials and Methods: The viability of human hepatoma (HepG2) cells exposed to radiation was evaluated by both the MTT and Trypan blue assays. The cells were seeded on 96 well-plates at a density of 1 x 104 cells/well, incubated overnight, and irradiated with...

The application of a tetrazolium salt, WST-8,2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt to the lymphocyte proliferation assay in the chicken system was evaluated. Proliferation of concanavalin (Con A)-induced splenic lymphocytes and peripheral blood lymphocytes (PBL) was evaluated with WST-8 and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte...

The hydrogen acceptor 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) is commonly utilized to estimate cellular viability in drug screening protocols. The present investigation was prompted, in part, by observations that reduction of MTT to its colored reaction product, MTT formazan, varied between cell lines and with culture age. A correlation was established between the D-g...

aim and background: hepatocellular carcinoma (hcc) -an aggressive malignancy with high rate of mortality- is the third cause of cancer related death, worldwide. cisplatin is a chemotherapy drug used for treatment of hcc. however, serious side effects of drug limit the therapeutic administer dose.  nanotechnology based devices such as liposome nanoparticles are capable to overcome these drawback...

nano-silver (agnp) has biological properties which are significant for consumer products, food technology, textiles and medical applications (e.g. wound care products, implantable medical devices, in diagnosis, drug delivery, and imaging). for their antibacterial activity, silver nanoparticles are largely used in various commercially available products. thus, the use of nano-silver is becoming ...

the anti-cancer activity of metal ions in the lanthanide group is being considered recently. it has been reported that cerium salts might stimulate the metabolism and therefore, produce anti-cancer effects. however, little is known about the effects of protein-cerium complex in controlling cancer cell growth. the aim of the present study was to elucidate the possible pathways for the cytotoxic ...