The performance of the PLP, log-RASTA-PLP, and J-RASTA-PLP front ends for recognition of highly reverberant speech is measured and compared with the performance of humans and the performance of an experimental RASTA-like front end on reverberant speech, and with the performance of a PLP-based recognizer trained on reverberant speech. While humans are able to reliably recognize the reverberant t...

An Overview on PLP-dependent Enzymes Pyridoxal 5’-phosphate (PLP), the biologically active of vitamin B6, was first identified in the mid-forties as the cofactor for the transamination reaction. Since then, PLP-dependent enzymes have been the focus of extensive biochemical research. The interest aroused by these enzymes is due to their unrivalled catalytic versatility and their widespread invol...

Markedly increased circulating concentrations of pyridoxal-5'-phosphate (PLP) were found in each of 14 patients representing all clinical forms of hypophosphatasia, an inborn error characterized by deficient activity of the tissue nonspecific (bone/liver/kidney) isoenzyme of alkaline phosphatase (AP). The mean PLP concentration in plasma was 1174 nM (range, 214-3839 nM) in the patients and 57 +...

Glucose 6phosphate dehydrogenase from streptomyces aureofaciens was purified and inactivated by pyridoxal 5′-phosphate (PLP). The inactivation was a pseudo-first order and time-dependent reaction. Complete inactivation was achieved at 0.2mM PLP within 16 minutes. The type of inhibition was competitive with respect to Glucose 6phosphate. Spectral characteristics of PLP-enzyme complex corresponde...

We cloned and expressed human pyridoxal-5\'-phosphate (PLP) phosphatase, the coenzymatically active form of vitamin B6, in Escherichia coli using pET15b vector. Monoclonal antibodies (mAb) were generated against purified human brain PLP phosphatase in mice, and four antibodies recognizing different epitopes were obtained, one of which inhibited PLP phosphatase. The binding affinities of these f...

BACKGROUND Paeonia lactiflora Pall. (PLP), a traditional Chinese herbal medicine, has been used for hepatic disease treatment over thousands of years. In our previous study, PLP was shown to demonstrate therapeutic effect on hepatitis with severe cholestasis. The aim of this study was to evaluate the antioxidative effect of PLP on alpha-naphthylisothiocyanate (ANIT)-induced cholestasis by activ...

background: l-tryptophan is used widespread in the pharmaceutical industry. the majority of l-trp production depends on microbial processes that produce l-tryptophan from indole and l-serine. these processes are very costly due to the costs of precursors, especially l-serine. use of inexpensive substitutions as the l-serine source of l-tryptophan production enables us to reach a cost-effective ...

Identifying and quantifying autoaggressive responses in multiple sclerosis (MS) has been difficult in the past due to the low frequency of autoantigen-specific T cells, the high number of putative determinants on the autoantigens, and the different cytokine signatures of the autoreactive T cells. We used single-cell resolution enzyme-linked immunospot (ELISPOT) assays to study, directly ex vivo...

Low vitamin B-6 status, based on plasma concentrations of pyridoxal-5-phosphate (PLP), has been identified in inflammatory diseases, including cardiovascular disease, rheumatoid arthritis, inflammatory bowel disease, and diabetes. Our objective was to examine the association between plasma PLP and multiple markers of inflammation in a community-based cohort [n = 2229 participants (55% women, me...

X-linked hereditary spastic paraplegias (HSPs) present with two distinct phenotypes: pure and complicated. The pure form is characterized by slowly progressive weakness and spasticity of the lower limbs, whereas the complicated forms have additional features (optic neuropathy, retinopathy, extrapyramidal disturbance, dementia, epilepsy, ataxia, ichthyosis, mental retardation, and deafness). Thr...