objective: in this study, we investigated the combined effect of 2-methoxyestradiol (2me2) and 60co on the cytogenetic damage of iododeoxyuridine (iudr) in the spheroid model of u87mg glioblastoma cancer cell lines by alkaline comet assay. materials and methods: u87mg cells were cultured as spheroids with diameters of 350 μm. as control, the spheroids of one plate were not treated. other cultur...

We present Gemini GMOS longslit spectroscopy of the isolated S0 galaxy NGC 3115. We have determined kinematical data and Lick/IDS absorption line-strength indices for the major axis out to around 9 kpc and for the minor axis out to around 5 kpc (around 2Re). Using stellar population models which include the effects of variable [α/Fe] ratios we derive metallicities, abundance ratios and ages for...

Microgravity induces three-dimensional (3D) growth in numerous cell types. Despite substantial efforts to clarify the underlying mechanisms for spheroid formation, the precise molecular pathways are still not known. The principal aim of this paper is to compare static 1g-control cells with spheroid forming (MCS) and spheroid non-forming (AD) thyroid cancer cells cultured in the same flask under...

We aim to identify levels of signal factors secreted by MSCs cultured in 2D monolayers (2D-MSCs), spheroids (spheroids MSCs), and cocultures of microvesicles (MVs) derived from 2D-MSCs or spheroid MSCs and retinal photoreceptor neurons. We seeded 2D-MSCs, spheroid MSCs, and cells derived from spheroids MSCs at equal numbers. MVs isolated from all 3 culture conditions were incubated with 661W ce...

After the discovery of functionally superior Tie2-positive nucleus pulposus (NP) progenitor cells, new methods were needed to enable mass culture and cryopreservation maintain these cells in an undifferentiated state with high cell yield. We used six types EZSPHERE® dishes, which support spheroid-forming colony culture, examined NP spheroid-formation ability, number, proliferation, mRNA express...

This work highlights the importance of surface modification on quality and quantity spheroid formation microfluidic-based biochips.

Abstract Spheroids have become essential building blocks for biofabrication of functional tissues. Spheroid formats allow high cell-densities to be efficiently engineered into tissue structures closely resembling the native In this work, we explore assembly capacity cartilaginous spheroids ( d ? 150 µ m) in context endochondral bone formation. The fusion at various degrees differentiation was i...

In this paper, we demonstrate formation of size-controlled 3-dimensional hetero-spheroids using a micro-fluidic device in approximately 2 min. The device collects cells in the center of a micro-chamber using micro-rotational flow as shown in Fig. 1. In previous work, we used this device to generate size-controlled HepG2 spheroids [1]. We applied it to form heterospheroids consisting of HepG2 an...

background: rad51 is a protein with critical role in double strand break repair. down-regulation of this protein has a significant effect in radiosensitivity of some cell lines like prostate carcinoma. compared to monolayer cell culture model, the spheroids are more resistant to radiation. the aim of the current study was to determine the rad51 protein level in du145 spheroids, and monolayer ce...