cumin (cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in iran. the purpose of this study was to evaluate the effect of plant growth regulators and explant type on callus induction in cumin. for this purpose the cumin seeds (kuhbanan accession) were disinfected with sodium hypochlorite and alcohol and cultured on ms basal medium. leaf and hyp...

Technique for rapid mass propagation of Geodorum densiflorum (Lam)Schltr. has been developed by using thin sections of stems of in vitro regenerated plantlets as explant source. Thin sections of stems (1.0-1.5 mm) when cultured in modified liquid and semisolid Knudson C (KnC) medium produced 1.8 and 1.2 protocorm like bodies (PLBs) per explant respectively. Peptone (2 g l-1) was effective in pr...

In the current study, the PL7 strain was isolated from soil and identified as Raoultella planticola based on its physiological characteristics and 16S rDNA sequence. By the 10th day, the PL7 strain degraded 52.0% of the pyrene (PYR) content and 50.8% of the benzo[a]pyrene (BaP) content in 20 mg L-1 PYR and 10 mg L-1 BaP in the liquid matrix. The half-life of PYR and BaP by PL7 degradation was 8...

Triploid plants of neem were obtained by immature endosperm culture. Immature seeds, at the early dicotyledonous stage of embryo development, is the best explant to raise endosperm callus on MS + NAA (5 mumol/L) + BAP (2 mumol/L) + CH (500 mg L-1). Maximum shoot bud differentiation from the endosperm callus occurred on MS + 5 mumol/L BAP. Shoots were multiplied by forced axillary branching and ...

A modifying influence of ortho-cresol (o-cresol) on the carcinogenic effect of benzo(a)pyrene (BaP) with combined oral administration to CC57Br mice had been found. During simultaneous administration of o-cresol (1 mg) and BaP (1 mg), the incidence of tumors, the multiplicity of tumors, and the degree of malignancy all increased, but the latency was shortened. When o-cresol was administered bef...

different explants of crocus sativus l. such as corm, style, ovary, petal, anther and leaf were cultured on ls and ms media supplemented with naa and bap or 2,4-d plus bap at different combinations. shoot and corm induction occurred in addition to the formation of other organs. efficient plantlet regeneration with strong roots occurred at 1 mg 1-i of both naa and bap. an increase in 2,4-d level...

The present study was the first report on somatic embryogenesis of Tunisian chickpea (Cicer arietinum L.) particularly supposed to be recalcitrant and difficult manipulate in vitro. An efficient protocol has been developed for inducing indirect derived from immature zygotic embryo axis, young leaflet hypocotylar explants. Callogenesis achieved full-strength Murashige Skoog's (1962) (MS) basal m...