was quantified by integration using EZLogicTM integration software (Bio-Rad). The clearance of host cell proteins (HCP) and double-stranded DNAs (dsDNA) was determined by a CHO-CM HCP ELISA kit (Cygnus Technologies) and Quant-iT dsDNA high-sensitivity assay kit (Life Technologies Corporation), respectively. mAb1 concentration was determined by UV absorption at 280 nm using a coefficient of 1.4 ...

The type VI secretion system (T6SS) is widely distributed in pathogenic Proteobacteria. Sequence and structural analysis of T6SS reveals a resemblance to the T4 bacteriophage tail, in which an outer sheath structure contracts an internal tube for injecting nucleic acid into bacterial cells. However, the molecular details of how this phage tail-like T6SS structure is assembled in vivo and execut...

AIM To explore the views of Malaysian healthcare professionals (HCPs) on stakeholders' decision making roles in localized prostate cancer (PCa) treatment. METHODS Qualitative interviews and focus groups were conducted with HCPs treating PCa. Data was analysed using a thematic approach. Four in-depth interviews and three focus group discussions were conducted between December 2012 and March 20...

BACKGROUND Burnout among healthcare professionals is one of the key challenges affecting health care practice and quality of care. This systematic review aims to (1) estimate the prevalence of burnout among health care professionals (HCP) in Arab countries; and (2) explore individual and work-related factors associated with burnout in this population. METHODS Multiple electronic databases wer...

Mobile genetic elements represent a large portion of the genome in many species. Posing a danger to the integrity of genetic information, silencing and structural machinery has evolved to suppress the mobility of foreign and transposable elements within the genome. Condensin proteins – which regulate chromosome structure to promote chromosome segregation – have been demonstrated to function in ...

Myopathy of insidious onset produced by corticosteroids is well recognised. In recent years acute myopathy has been reported in patients with acute severe asthma treated with 3-4 g of intravenous hydrocortisone a day.1 2 It has been argued that to minimise the risks of this the dose should be no more than 1 g of hydrocortisone a day.2 There is evidence that 1 g/day is an effective regimen.3 We ...

OBJECTIVES Low cardiac output syndrome is common after paediatric cardiac surgery. Previous studies suggested that hydrocortisone administration may improve haemodynamic stability in case of resistant low cardiac output syndrome in critically ill children. This study was set up to test the hypothesis that the effects of hydrocortisone on haemodynamics in children with low cardiac output syndrom...

1 The concept that nitric oxide (NO) release can be beneficial in inflammatory conditions has raised more attention in the recent years, particularly with the development of nitric oxide-releasing anti-inflammatory drugs. There is considerable evidence that NO is capable of enhancing the anti-inflammatory benefits of conventional anti-inflammatory drugs. 2 Since hydrocortisone is the most widel...

CONTEXT Patellofemoral pain syndrome (PFPS) is a common orthopaedic condition for which operative and nonoperative treatments have been used. Therapeutic modalities have been recommended for the treatment of patients with PFPS-including cold, ultrasound, phonophoresis, iontophoresis, neuromuscular electrical stimulation, electrical stimulation for pain control, electromyographic biofeedback, an...

bacground: evidence from several lines of investigations suggests that toll-like receptor 4 (tlr4) is involved in atherosclerosis as a bridge between innate and acquired immunity. percutaneous coronary intervention (pci) can trigger inflammation through activation of human tlr4 (htlr4) on monocytes. hydrocortisone as an anti-inflammatory and immuno-suppressant agent has multiple mechanisms of a...