The binding curves of histones H1 and H5 to chromatin in nuclei have been determined by a novel method which utilises the differential properties of free and bound histones on cross-linking with formaldehyde. The dissociation is thermodynamically reversible as a function of [NaCl]. The binding curves are independent of temperature over the range 4 degrees - 37 degrees C and independent of pH ov...

Experiments with antibodies induced by separated fragments 1-58 and 63-125 of H2B histone indicated that the 1-58 portion of the molecule is much more accessible in chromatin than is the 63-125 region. In immunoabsorption and immunoelectron microscopic assays with bovine and chicken chromatins, anti-1-58 antibodies reacted with sheared or unsheared chromatin both at low ionic strength (1 mM Tri...

Single-walled carbon nanotubes (SWNTs) can be dispersed as individuals in H2O using sodium dodecylsulfate (SDS), typically at 1% (35 mM) concentration. At and above the critical micellar concentration (cmc 8 mM at 25 °C), the degree of ionization of SDS is 0.27.1 Once intertube van der Waals (VdW) attraction is overcome by intense sonication, free SDS adsorbs to SWNT surfaces and creates a net ...

Highly concentrated NaCl brines are important geothermal fluids; chloride complexation of metals in such brines increases the solubility of minerals and plays a fundamental role in the genesis of hydrothermal ore deposits. There is experimental evidence that the molecular nature of the NaCl–water system changes over the pressure–temperature range of the Earth’s crust. A transition of concentrat...

The complete purification of malate dehydrogenase (EC 1.1.1.37) from extremely halophilic bacteria of the Dead Sea is described. The purification procedure includes (a) precipitation by ammonium sulfate, (b) fractionation on Sepharose 4B using a decreasing concentration gradient of ammonium sulfate, (c) gel permeation chromatography on Sephadex G-100, (d) chromatography on hydroxylapatite, and ...

OBJECTIVES To characterize the chromosome-encoded metallo-β-lactamase (MBL) from the psychrophilic, marine fish-pathogenic bacterium Aliivibrio salmonicida LFI1238 and check for the presence of the gene in other Aliivibrio isolates both connected to the fish-farming industry and from the environment. METHODS The MBL gene was cloned and intracellularly expressed in Escherichia coli. Kinetic pa...

Bandeiraea simplicifolia plant seeds contain a family of five alpha-D-galactopyranosyl-binding isolectins (BS I-A4, A3B, A2B2, AB3, B4) and N-acetyl-D-glucosamine-binding lectin (BS II). After Pi/NaCl extraction and ammonium sulfate fractionation BS II is specifically adsorbed onto p-aminobenzyl-1-thio-N-acetyl-beta-D-glucosaminide-succinylaminohexylaminyl--Sepharose-4B. The BS I isolectins pas...