In this study, we report the synthesis of two new copper complexes: [Cu(C11H7O2)(SCN)(C10H8N2)], denoted as (C-1), and [Cu(C11H7O2) (C12H8N2) Cl]·H2O, (C-2). They are based on 2,2′-bipyridine or 1,10-phenanthroline 2-hydroxy-1-naphtaldehyde ligands. The obtained complexes were characterized by FT-IR, UV-visible spectroscopy, single-crystal X-ray diffraction analysis. Molecular docking was emplo...

Optimization of the fermentation medium for maximum alkaline protease production was carried out. Fifteen positive isolates were examined for their extent of alkaline protease production. The most potent producer was identified as Bacillus sp. The solid substrate screening showed that the combination of wheat straw and bean husk was the best one. The initial screening by using Plackett–Burman’s...

Abstract: Coxsackievirus B3 (CVB3) is the most common agent known to cause viral myocarditis. The viral genome encodes a single polyprotein that is cleaved to produce several proteins by virally encoded proteases. Most of this proteolytic processing is catalyzed by a cysteine protease called 3C. The 3C protease plays major role in viral replication and cellular damage. To understand the mecha...

UNLABELLED Resistance to various human immunodeficiency virus type 1 (HIV-1) protease inhibitors (PIs) challenges the effectiveness of therapies in treating HIV-1-infected individuals and AIDS patients. The virus accumulates mutations within the protease (PR) that render the PIs less potent. Occasionally, Gag sequences also coevolve with mutations at PR cleavage sites contributing to drug resis...

background: one of the most important producers of high quality industrial enzymes is the gram-positive bacterium, bacillus subtilis (b. subtilis). one major limitation that hinders the wide application of b. subtilis is the secretion of high levels of extracellular proteases which degrade the secreted foreign proteins. in this study, homologus recombination technique was used to knock out its ...

background: considering their specific adaption to marine milieus, marine actinomycetes constitute an excellent source for producing unique bioactive substances. they are also important sources of antibiotics and extracellular enzymes, geosmin, and enzymes with potent antibacterial and anticarcinogenic properties and low toxicity. the objective of this study is to isolate actinomycetes producin...

The protease enzyme produced by Lactic Acid Bacteria (LAB) with characteristics similar to rennin is called Rennin Like Protease (RLP). purpose of the study was obtain information on biochemical RLP Enterococcus faecium 1.15 as a basis for utilization enzyme. first stage observation growth curves and potential isolates produce RLP, characterization. (RLP) which has been purified using Sephadex ...

Protease widely exists in the digestive tract of animals and humans, playing a very important role in protein digestion and absorption. In this study, a high protease-producing strain Planomicrobium sp. L-2 was isolated and identified from the digestive tract of Octopus variabilis. The strain was identified by physiological and biochemical experiments and 16S rDNA sequences analysis. A protease...

A real-time fluorescence turn-on strategy for protease activity and inhibitor screening has been developed. A negatively charged benzo[ghi]perylene derivative (probe 1) was employed. Protamine is a cationic protein which can induce aggregation of probe 1 via strong electrostatic and hydrophobic interactions. The fluorescence of probe 1 was efficiently quenched. In the presence of a protease, pr...