Three fractions of choline O-acetyltransferase (ChAT) (EC 2.3.1.6) were solubilized from a nerve ending fraction of rat forebrain using three sequential washes of an increasingly chaotrophic nature (100 mM sodium phosphate, pH 7.4; 500 mM NaCl; 2% Triton DN-65) as previously described (Benishin, C.G., and P.T. Carroll (1983) J. Neurochem. 41: 1030-1039). The molecular weights of the soluble (Na...

The mechanism by which plants cope with salt stress remains poorly understood. The goal of this study is to systematically investigate the contribution and distribution of inorganic ions and organic compounds to the osmotic adjustment (OA) in the halophyte species Halostachys caspica. The results indicate that 100-200 mM NaCl is optimal for plant growth; the water content and degree of succulen...

In this study, it was aimed to investigate the effects of salinity, which is an important environmental problem, in cultivation ornamental plants (such as zinnia) and irrigation with high salt water, especially on antioxidant defense mechanism. For purpose, two Zinnia species were irrigated by different concentrations saline water (50, 100, 150, 200 mM NaCl); salinity superoxide dismutase (SOD)...

The potential role of NaCl (50–200 mM) as an eustressor for the accumulation health promoting phytochemicals and maintaining homeostasis macro- micro-elements in three, hydroponically grown Brassica leafy vegetables (Chinese cabbage, white kale) was investigated. Considering K+/Na+ ratio proline contents reliable stress markers, we confirmed more prominent status Chinese cabbage followed by kal...

to study the interaction effect of the salinity and salicylic acid on some morphological and physiological traits in clary sage (salvia sclarea), a pot experiment was conducted as factorial based on a completely randomized design with three levels of nacl and control (zero, 50, 100 and 150 mm), as the first factor and three levels of spraying the solution of salicylic acid and control (zero, 10...

Aqueous dispersions of 50 mM dimyristoylphosphatidylglycerol (DMPG) in the presence of increasing salt concentrations (2-500 mM NaCl) were studied by small angle X-ray scattering (SAXS) and optical microscopy between 15 and 35 degrees C. SAXS data show the presence of a broad peak around q approximately 0.12 A(-1) at all temperatures and conditions, arising from the electron density contrasts w...

hybrid populations (f3) derived from cross between four moderate salt-resistant spring wheat (triticum aestivum l.) were assessed for transgressive segregation of salt-tolerant genotypes allowing germination and emergence at 350 mm nacl for 30 days. the achieved selection intensity was the order of 0.24-0.27 in these segregation populations (22-25 seedlings from 9000 seeds). assessment of trans...

the effects of salinity and supplied calcium chloride on growth and leaf ions concentration of tomato (lycopersicon esculentum l.) were investigated in gorgan, iran. a factorial experiment was conducted based on rcbd with four nacl levels (0, 50, 100, and 150 mm) and four cacl2 levels (0, 100, 200 and 300 mg l-1). data of growth, yield and leaf’s ca, k, and na content were subjected to analyze ...

Protein constructs and purification For purification of NFκB:IRF-7:IRF-3, a freshly transformed colony was transferred into 10 ml LB broth containing 100 μg/ml ampicillin and grown overnight at 37°C to saturation. This overnight culture was used to inoculate 1 liter LB broth containing 100 μg/ml ampicillin and grown at 37°C to an OD600 of about 0.5. The culture was then induced with 0.4 mM isop...