Full genome sequencing of bacterial genomes has revealed the presence of numerous genes encoding family X DNA polymerases. These enzymes play a variety of biological roles and, accordingly, display often striking functional differences. Here we report that the PolX from the heat-stable organism Thermus thermophilus (TthPolX) inserts the four dNTPs with strong asymmetry. We demonstrate that this...

Table 1: Accuracy on small datasets (in %). Validation RF ET P-DT P-ET RS-DT RS-ET RP-DT RP-ET diabetes 77.12 (6) 77.25 (5) 77.67 (4) 78.01 (3) 75.11 (8) 76.77 (7) 78.82 (2) 79.07 (1) dig44 94.99 (7) 95.78 (1) 91.86 (8) 95.46 (4) 95.07 (6) 95.69 (3) 95.13 (5) 95.72 (2) ionosphere 94.40 (6) 95.15 (3) 93.86 (8) 94.75 (5) 94.11 (7) 94.90 (4) 95.20 (2) 95.36 (1) pendigits 98.94 (7) 99.33 (1) 98.09 ...

T7 phage RNA polymerase was used to transcribe a series of DNA templates bearing any of several precisely localized lesions. Lesions were positioned downstream of the T7 promoter on either strand of the DNA template to investigate the effects of these lesions on elongation of transcription. The following four types of DNA modifications were studied: 1) 3-hydroxy-2-hydroxymethyltetrahydrofuran (...

1 The authors thank CAIDA for providing this set of traffic traces. Additional information for these traces can be found in the DatCat, Internet Measurement Data Catalog [8], indexed under the label ‘‘PAIX’’. 1910 M. Iliofotou et al. / Computer Networks 55 (2011) 1909–1920 behavior. Flow-level and payload-based classification methods require per application training and will thus not detect P2P...

Replication of DNA containing 7,8-dihydro-8-oxo-2'-deoxyguanosine (OxodG) gives rise to G --> T transversions. The syn-isomer of the lesion directs misincorporation of 2'-deoxyadenosine (dA) opposite it. We investigated the role of the 2-amino substituent on duplex thermal stability and in replication using 7,8-dihydro-8-oxo-2'-deoxyinosine (OxodI). Oligonucleotides containing OxodI at defined ...