A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. The ...

barium salts of dibenzylidene cystines 8a-c were obtained from the reaction of lcystine with benzaldehyde or its derivatives in the presence of barium hydroxide. their reduction with zinc and hydrochloric acid in methanol yielded the narylmethylcysteines ?9a-c?. these can, in turn, be converted by esterification into the methyl esters ?10 a-c. ?reaction of n, n'-carbonyl diimidazole (im co...

The front cover picture, designed by Tsuchimoto, illustrates the world of formation o-bis(3-indolyl)arenes that we had not anticipated. Before starting this research project, our purpose was to develop a unique-forming process 3-arylindoles reacting indoles with 7-oxabicyclo[2.2.1]hept-5-en-2-one in presence (Lewis) acid catalyst and H−Si. Contrary expectations, treatment two substrates CF3SO3H...