The aim of the present study is to delineate the role of human chorionic gonadotropin (hCG) in trophoblast fusion. In this direction, using shRNA lentiviral particles, α- and β-hCG silenced 'BeWo' cell lines were generated. Treatment of both α- and β-hCG silenced BeWo cells with either forskolin or exogenous hCG showed a significant reduction in cell fusion as compared with control shRNA treate...

To determine whether injection of hCG or GnRH-agonist on d 5 after estrus (d 0) has a differential functional effect on an induced and the original corpus luteum (CL), two experiments were conducted. In Exp. 1, nonlactating Holstein cows were injected on d 5 with saline (n = 4; T1), a GnRHagonist (Buserelin, 8 mg i.m.; n = 4; T2), or hCG (1,000 IU, i.v., and 2,000 IU, i.m.; n = 4; T3). Induced...

Objective. Morphologically, β-HCG secreting somatic carcinoma can be difficult to distinguish from epithelioid trophoblastic tumors (ETT). However, their distinction is critical due to their potentially differing prognoses and choice of chemotherapy. Presence of biparental alleles in ETT can be identified with molecular testing. We describe a patient who presented with metastatic carcinoma and ...

The pregnancy hormone human chorionic gonadotropin (hCG) is also present at low concentrations in plasma and urine of men and nonpregnant women. hCG immunoreactivity occurs in various molecular forms: Besides the intact hCG heterodimer, considerable amounts of proteolytically cleaved forms, free subunits, and fragments are found in plasma and urine. Especially in urine, proteolytic fragments co...

The nature of the substance with thyroid-stimulating activity (TSA) present in human chorionic gonadotropin (hCG) prepared from pregnancy urine was investigated. In the mouse thyrotropin bioassay, the characteristic maximum of blood radioactivity obtained with the TSA in hCG preparations occurred after that obtained with pituitary thyrotropin (hTSH) but before that obtained with long-acting thy...

The placenta may be involved in the regulation of the maternal thyroid function during pregnancy. As human chorionic gonadotropin (hCG) is a primary protein from the placenta, we examined the thyrotropic activity of hCG and its derivatives using tissue culture and monolayer cell culture of human thyroid glands. The TSH receptor preparation was made from thyroid tissues, and its binding affinity...

Trisomy 21 is associated with high maternal serum concentrations of intact human chorionic gonadotrophin alpha(HCG) and free beta-HCG whereas these concentrations are markedly decreased in trisomy 18. In this study, we investigated the effect of trisomy 21 and 18 on endogenous HCG concentrations and luteinizing hormone (LH)/HCG receptor expression in placental villous tissue in eight trisomy 21...

The isolated cell membranes from bovine corpora lutea bound lz51-human chorionic gonadotropin (HCG) with high affinity and specificity. The specific binding was saturable with respect to the amounts of membrane protein and 12jIHCG added. Approximately 8.4 x 10-l” M 124-HCG saturated all the receptor sites in 1.0 mg of membrane protein. The specific binding of 12sI-HCG was strong and not readily...

Volume 4 • Issue 2 • 1000e124 JFIV Reprod Med Genet ISSN: 2375-4508 JFIV, an open access journal In vitro oocyte maturation (IVM) has carved a niche for itself in assisted reproductive technology (ART). In 1991, Cha et al. [1] reported the first collection of immature oocytes, which were matured in vitro and resulted in a live birth. Since then, IVM protocols have developed ranging from IVM wit...

Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) are glycoprotein hormones used for assisted reproduction acting on the same receptor (LHCGR) and mediating different intracellular signaling. We evaluated the pro- and anti-apoptotic effect of 100 pM LH or hCG, in the presence or in the absence of 200 pg/mL 17β-estradiol, in long-term, serum-starved human primary granulosa cells (h...