This study examined the association between 17p allelic loss and p53 gene mutation in a series of 16 esophageal adenocarcinomas arising on a background of Barrett's esophagus. Two highly polymorphic dinucleotide repeat polymorphisms mapping to 17p13 were analyzed to assess the frequency of 17p allelic loss in these tumors. Mutations in the p53 gene were detected by direct DNA sequencing. Ninety...

Molecular characterization of Candida albicans isolates is essential for understanding the epidemiology of nosocomial infections caused by this yeast. Here, we investigated the potential value of multilocus sequence typing (MLST) for characterizing epidemiologically related or unrelated C. albicans strains of various clinical origins. Accordingly, we sequenced the internal regions (loci) of six...

  In an attempt to characterize Theileria parva parasites circulating in South Sudan cattle , polymerase chain reaction (PCR)-based assays were carried out using four single copy encoding antigen genes p104, PIM, p150 and p67 in addition to one microsatellite MS321. A total of 20 bovine DNA samples from two locations in South Sudan were included in this study, in addition to two references stra...

Using an (AG)13 enriched genomic library of Mugil cephalus, 12 polymorphic microsatellite loci were isolated and characterized in a test population; the number of alleles ranged from 2 to 11. The observed and expected heterozygosities ranged from 0.2593 to 0.8966 and from 0.3047 to 0.8454, respectively. Two loci deviated from Hardy-Weinberg equilibrium; linkage disequilibrium among the 12 loci ...

objective(s): short tandem repeat (str) loci are the most informative dna genetic markers for attempting to individualize biological material for application in paternity and forensic cases. materials and methods: blood samples were collected and the total genomic dna was extracted. the dna samples were used for genotyping vwa and tpox str loci using pcr and polyacrylamide gel electrophoresis. ...

We present primers and amplification conditions for 15 microsatellite loci developed for the Cope's giant salamander (Dicamptodon copei), 14 of which are tetranucleotide repeats. Cross-species amplification revealed 10 of these loci to also be polymorphic in the Pacific giant salamander (Dicamptodon tenebrosus). Several loci produced nonoverlapping allelic ranges between the two species and may...

‘Žilavka’ has been grown in Bosnia and Herzegovina since the XIVth century and is exploited for wine production. Although not sufficiently studied, this grapevine cultivar has high economic potential for the country. Five survey missions resulted in the collection of eighty ‘Žilavka’ accessions that varied in terms of names and characteristics. Because of the unknown origin of the cultivar, the...

Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic ...

The catadromous Platyeriocheir formosa is a crab endemic in Taiwan. To conserve P. formosa population diversity and ensure the sustainable use of this natural resource, we have developed new genetic markers, 17 polymorphic microsatellite loci, to promote the study of its population genetics in the future. In this study, more than 70 microsatellite sequences were found. Among these, 18 loci were...