نتایج جستجو برای: 5 elisa
تعداد نتایج: 1253905 فیلتر نتایج به سال:
Through the present study, we produced a monoclonal antibody against aflatoxin B1 (AFB1) using AFB1- carboxymethoxylamine BSA conjugates. One clone showing high binding ability was selected and it was applied to develop a direct competitive ELISA system. The epitope densities of AFB1-CMO against BSA and KLH were about 1 : 6 and 1 : 545, respectively. The monoclonal antibody (mAb) from cloned hy...
A selective pressurized liquid extraction (SPLE) method was developed for a streamlined sample preparation/cleanup to determine Aroclors and coplanar polychlorinated biphenyls (PCBs) in soil and sediment. The SPLE was coupled with an enzyme-linked immunosorbent assay (ELISA) for an effective analytical approach for environmental monitoring. Sediment or soil samples were extracted with alumina, ...
Dot-ELISA using the outer membrane complex antigens of Neisseria meningitidis as a target was standardized for rapid detection of meningococcal-specific antibodies in human serum. We investigated the level of meningococcal-specific IgG, IgA, and IgM in serum using dot-ELISA with outer membrane antigens prepared from Neisseria meningitidis serotype B:4.19:P1.15,3,7,9 (a strain isolated from a Br...
BACKGROUND The objectives of this study were to determine the proportion of malaria, bacteraemia, scrub typhus, leptospirosis, chikungunya and dengue among hospitalized patients with acute undifferentiated fever in India, and to describe the performance of standard diagnostic methods. METHODS During April 2011-November 2012, 1564 patients aged ≥5 years with febrile illness for 2-14 days were ...
BACKGROUND Breast cancer is the most common malignancy with the highest incidence rates among women worldwide. Triple-negative breast cancer (TNBC) disease is diagnosed more frequently in younger women, and is associated with a poor prognosis. Elevated levels of serum haptoglobin protein (Hp) are observed in many malignant diseases including breast cancer. We evaluated the expression and progno...
سابقه و هدف: بیماران مبتلا به لوسمی میلوئیدی مزمن (cml) دارای کرموزوم فیلادلفیا هستند. این کروموزوم حاصل جابجایی بازوهای بلند کروموزوم های 9 و 22 (q34q11) بوده که منجر به ایجاد الحاق بین ژنهای bcr و abl می گردد. در این مطالعه، تکنیک های rt-pcr و nasba در تعیین رونویس های bcr-abl مقایسه شد.روش بررسی: رونویس های الحاقی بطور مصنوعی سنتز و rna از رده سلولی لوسمیک k562 استخراج گردید. سریال رقت هم از...
background: toxoplasma gondii , the obligate intracellular parasite is life threatening in aids patients. diagnosis of toxoplasmosis is based on serological methods especially increasing of igm and igg titers, but finding of parasite or its components (antigenemia) may be beneficial method in order to detection of acute toxoplasmosis in immunocompromised patients. methods: ninety-four serum sam...
Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate...
BACKGROUND Feline immunodeficiency virus (FIV) infection is an important cause of disease of cats worldwide. Initial screening is commonly performed by commercially available point-of-care (POC) ELISA tests. Confirmatory testing for positive POC test results is recommended. Polymerase chain reaction (PCR) tests for FIV are commonly used additional testing methods; however, reported measures of ...
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