A 4-factor, multilevel, full factorial design of 240 experiments was performed in order to investigate the effect of temperature on the inactivation efficiency of spiked Escherichia coli in simulated solar disinfection of a synthetic secondary effluent. The initial population of the microorganisms was 10, 10, 10 and 10 CFU/mL, the exposure time 1, 2, 3 and 4 h, the treatment temperature 20, 30,...