Reverse gyrase is a peculiar DNA topoisomerase, specific of hyperthermophilic Archaea and Bacteria, which has the unique ability of introducing positive supercoiling into DNA molecules. Although the function of the enzyme has not been established directly, it has been suggested to be involved in DNA protection and repair. We show here that the enzyme is degraded after treatment of Sulfolobus so...

In the present study, the Allium cepa chromosome assay was employed as a preliminary test to investigate the mutagenic and antimutagenic potential of three plants, namely Clinacanthus nutans, Adhatoda vasica, and Carica papaya, used by traditional practitioners in Malaysia against a direct acting mutagen-Methyl Methanesulphonate (MMS). Onions were planted in various treatment groups: plant extr...

Monofunctional alkylating agents like methyl methanesulfonate (MMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) are potent inducers of cellular stress leading to chromosomal aberrations, point mutations, and cell killing. We show that these agents induce a specific cellular stress response program which includes the activation of Jun N-terminal kinases/stress-activated protein kinases (JNK/...

© 2012 The Authors. doi: 10.2340/00015555-1264 Journal Compilation © 2012 Acta Dermato-Venereologica. ISSN 0001-5555 Spitz nevus (SN) was first described in 1948, and was referred to as melanoma of childhood (1). Although SNs are benign melanocytic neoplasms, these lesions share many histopathological findings with malignant melanomas (MMs) (2). Despite the diagnostic criteria, the similarities...

Various applications have utilized a mobile mapping system (MMS) as the main 3D urban remote sensing platform. However, the accuracy and precision of the three-dimensional data acquired by an MMS is highly dependent on the performance of the vehicle’s self-localization, which is generally performed by high-end global navigation satellite system (GNSS)/inertial measurement unit (IMU) integration...

BACKGROUND We hypothesized that the use of oxygen supplementation during aerobic exercise induces less DNA damage than exercise alone. The aim of this study is to assess the level of DNA damage induced by physical exercise with and without oxygen supplementation in chronic obstructive pulmonary diseases (COPD) patients. METHODS Peripheral blood was collected before and after aerobic exercise ...

Yeast Rad27 is a 5'-->3' exonuclease and a flap endo-nuclease. Apn1 is the major apurinic/apyrimidinic (AP) endonuclease in yeast. The rad27 deletion mutants are highly sensitive to methylmethane sulfonate (MMS). By examining the role of Rad27 in different modes of DNA excision repair, we wish to understand why the cytotoxic effect of MMS is dramatically enhanced in the absence of Rad27. Base e...

The effect of mixed mutagen exposures on the rate and type of induced mutants was studied in the L5178Y/TK+/-----TK-/- mouse lymphoma cell mutagenicity assay. In this assay, exposure to ethyl methanesulfonate (EMS) results in more mutants that form large colonies than small colonies. Exposure to methyl methanesulfonate (MMS) results in more mutants that form small colonies than large colonies. ...

MMS induces diverse rII mutations from a wild-type background in bacteriophage T4. About 56% are base pair substitutions, about 30% are frameshift mutations, and the remainder is a miscellaneous set of rapidly reverting or leaky mutants of unknown composition; but deletions were not detected. MMSinduced forward mutation is sharply reduced by the mutations px and y, which also reduce ultraviolet...

Sebaceous carcinomas (SC) are rare adnexal tumors with possible aggressive behavior usually arising in the head and neck region of adults in the seventh decade of life. Treatment has traditionally been with surgical excision with 5-6 mm wide margins but Mohs micrographic surgery (MMS) has also been reported as an effective treatment modality. We present a case of a Caucasian female renal transp...