-D-Amino acid oxidase has been identified within the granule fraction of human neutrophilic leukocytes. Leukocyte homogenates and purified kidney 1-amino acid oxidase can utilize either isolated D-amino acids or some species of bacteria as substrates for the generation of hydrogen peroxide. When linked to leukocyte myeloperoxidase in vitro, purified D-amino acid oxidase constitutes a system let...

DNA damage by oxidative stress is one of the causes of mutagenesis. However, whether or not DNA damage induces illegitimate recombination has not been determined. To study the effect of oxidative stress on illegitimate recombination, we examined the frequency of lambdabio transducing phage in the presence of hydrogen peroxide and found that this reagent enhances illegitimate recombination. To c...

background: there have been numerous researches on ozone application in dentistry; yet the data regarding its whitening effect is very limited. the present study compares the bleaching effect of ozone with offi ce bleaching. materials and methods: in this experimental study, 15 maxillary premolar teeth were selected and sectioned mesio-distally and bucco-lingually. the sections were then placed...

The aim of the present experiments was to discern if the “entanglement”-like photon emissions from pairs of cell cultures or human brains separated by significant distances but sharing the same circling magnetic field could be demonstrated with a classic chemiluminescent reaction produced by hydrogen peroxide and hypochlorite. Simultaneous injection of the same amount of peroxide into a local d...

To determine the types of mutations induced by oxidative damage, a kidney cell line with a heterozygous deficiency for the autosomal Aprt (adenine phosphoribosyltransferase) gene was tested for its mutagenic response to hydrogen peroxide. Aprt-deficient cells were selected and scored for loss of heterozygosity (LOH) for 11 microsatellite loci on mouse chromosome 8. On the basis of the LOH analy...

hydrogen peroxide, an oxidizing agent used in surgery for its antiseptic properties, has been associated with life-threatening complications. we report a case of suspected gas embolism following irrigation of a tibial bone cyst with hydrogen peroxide in a five year-old boy under general anesthesia, who died despite resuscitative measures and a long stay in the intensive care unit.

The formation of hydrogen peroxide during the oxidation of NADH by purified preparations of cytochrome o has been demonstrated by employing three independent methods: polarographic, calorimetric, and fluorometric. The first two methods were used to assay for the accumulation of hydrogen peroxide and showed that hydrogen peroxide did accumulate as a product, but only about 30% of the oxygen cons...

the construction and performance of an amperometric microbiosensor for the determination of choline chloride by immobilizing choline oxidase on a cellulose acetate membrane are described. the procedure of choline determination involved the electrochemical detection of enzymatically generated hydrogen peroxide. this microsensor has a linear response between 0.038-0.1 mm choline chloride. the res...