In this study, we present the identification and characterization of hamster and guinea pig nicotinic acid receptors. The hamster receptor shares approximately 80-90% identity with the nucleotide and amino acid sequences of human, mouse, and rat receptors. The guinea pig receptor shares 76-80% identity with the nucleotide and amino acid sequences of these other species. [(3)H]nicotinic acid bin...

In article, we have reported a thermodynamic based study of aqueous solvation benzoic acid (C6H5COOH) at 288 to 318 Kelvin temperature. At this temperature range the dissociation constant (Ka) into solvent has been determined by applying titration method against standard basic solution NaOH different ionic strength NaCl. Although, in observation, value Ka is being inversely proportional respect...

to day, the activated carbon is being used to treat the industrial and domestic wastewaters. the suitability of activated carbon for treatment of industrial wastewaters can be quickly predicted by consideration of wastewater characteristics. the results of this paper represent a first step in determining suitability of activated carbon for treatment of wastewaters or effluent of treatment plant...

The binding of bilirubin to bovine serum albumin has been determined to be a second order process, first order in each reactant. Formation of the bilirubin . albumin complex was observed as an increase in absorbance at 480 nm by stopped flow spectrophotometry. The effect of long chain fatty acid on the binding of bilirubin was investigated at palmitic acid levels of 0 to ‘7 mollmol of albumin a...

The binding of bilirubin to bovine serum albumin has been determined to be a second order process, first order in each reactant. Formation of the bilirubin . albumin complex was observed as an increase in absorbance at 480 nm by stopped flow spectrophotometry. The effect of long chain fatty acid on the binding of bilirubin was investigated at palmitic acid levels of 0 to ‘7 mollmol of albumin a...

A graph is presented which permits facile determination of the dissociation constant for a macromolecule-ligand complex from gel filtration results; it can be used without a detailed understanding of the remainder of the text. When a solution of macromolecule and radioactive ligand is subjected to gel filtration, the amount of ligand associated with the eluted macromolecule is easily measured. ...

The binding of bilirubin to bovine serum albumin has been determined to be a second order process, first order in each reactant. Formation of the bilirubin . albumin complex was observed as an increase in absorbance at 480 nm by stopped flow spectrophotometry. The effect of long chain fatty acid on the binding of bilirubin was investigated at palmitic acid levels of 0 to ‘7 mollmol of albumin a...

The binding of bilirubin to bovine serum albumin has been determined to be a second order process, first order in each reactant. Formation of the bilirubin-albumin complex was observed as an increase in absorbance at 480 nm by stopped flow spectrophotometry. The effect of long chain fatty acid on the binding of bilirubin was investigated at palmitic acid levels of 0 to 7 mol/mol of albumin at 3...

The kinetics of ATP binding and hydrolysis (formation of acid-labile phosphate) by the Tetrahymena 30 S dynein ATPase has been measured by chemical quench flow methods. The amplitude of the ATP-binding transient gave a molecular weight per ATP-binding site of approximately 750,000, suggesting nearly 3 ATP binding sites/2 million Mr dynein molecule (Johnson, K. A., and Wall, J.S. (1983) J. Cell ...

The human protein Rap1A (Rap) is a member of the Ras superfamily of GTPases that binds to the downstream effector Ral guanine nucleotide dissociation stimulator (RalGDS). Although Ras and Rap have nearly identical amino acid sequences and structures along the effector binding surface, the charge reversal mutation Rap K31E has previously been shown to increase the dissociation constant of Rap-Ra...